Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance Mechanism
<i>Pichia kudriavzevii</i> is one of the major non-<i>Saccharomyces cerevisiae</i> yeasts in Chinese <i>baijiu</i> brewing, which has shown a substantially higher tolerance to acid, heat, and ethanol. Exploring the mechanism of <i>P. kudriavzevii</i> c...
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MDPI AG
2023-06-01
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| Series: | Fermentation |
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| author | Ning Wang Puyu Zhang Xiaoli Zhou Jia Zheng Yi Ma Chenguang Liu Tao Wu Hong Li Xiaoqin Wang Hong Wang Xudong Zhao Muhammad Aamer Mehmood Hui Zhu |
| author_facet | Ning Wang Puyu Zhang Xiaoli Zhou Jia Zheng Yi Ma Chenguang Liu Tao Wu Hong Li Xiaoqin Wang Hong Wang Xudong Zhao Muhammad Aamer Mehmood Hui Zhu |
| author_sort | Ning Wang |
| collection | DOAJ |
| description | <i>Pichia kudriavzevii</i> is one of the major non-<i>Saccharomyces cerevisiae</i> yeasts in Chinese <i>baijiu</i> brewing, which has shown a substantially higher tolerance to acid, heat, and ethanol. Exploring the mechanism of <i>P. kudriavzevii</i> could have a positive effect on the artificially controlled production of <i>baijiu</i>. In this study, an efficient acetic-acid-tolerant <i>P. kudriavzevii</i> strain, Y2, was isolated from the yellow water of strong-flavored <i>baijiu</i> brewing waste, and its molecular mechanism of acetic acid tolerance was investigated through a comparative transcriptomic analysis. The strain Y2 could tolerate 12 g/L of acetic acid. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, differentially expressed genes (DEGs) were mainly enriched in oxidative phosphorylation, the citrate cycle, glycolysis/gluconeogenesis, and carbon metabolism under low (AL group) and high (AH group) concentrations of acetic acid. However, the DEG enrichment was more profound in the AH group when compared to the control. Compared with the AL group, the expression of genes related to oxidative phosphorylation was more significantly upregulated, while in terms of the TCA cycle, phosphoenolpyruvate carboxykinase was significantly upregulated in both the AH and AL groups and was positively correlated with tolerance to acetic acid. This was followed by citrate synthase, isocitrate dehydrogenase, malate dehydrogenase, and succinate dehydrogenase. These results illustrated a possible mechanism of acid tolerance by regulating the metabolism-related pathways in <i>P. kudriavzevii</i> and provided a basis for the further investigation of the acid tolerance mechanism. |
| first_indexed | 2024-03-11T02:29:59Z |
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| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T02:29:59Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-b21bd1b73adc4d5396e203afe95d77ac2023-11-18T10:21:09ZengMDPI AGFermentation2311-56372023-06-019654010.3390/fermentation9060540Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance MechanismNing Wang0Puyu Zhang1Xiaoli Zhou2Jia Zheng3Yi Ma4Chenguang Liu5Tao Wu6Hong Li7Xiaoqin Wang8Hong Wang9Xudong Zhao10Muhammad Aamer Mehmood11Hui Zhu12College of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaWuliangye Group Co., Ltd., Yibin 644007, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaState Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Food and Biological Engineering, Xihua University, Chengdu 610039, ChinaWuliangye Group Co., Ltd., Yibin 644007, ChinaWuliangye Group Co., Ltd., Yibin 644007, ChinaWuliangye Group Co., Ltd., Yibin 644007, ChinaYibin Energy Conservation and Environmental Protection Industry Association, Yibin 644007, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong 643000, China<i>Pichia kudriavzevii</i> is one of the major non-<i>Saccharomyces cerevisiae</i> yeasts in Chinese <i>baijiu</i> brewing, which has shown a substantially higher tolerance to acid, heat, and ethanol. Exploring the mechanism of <i>P. kudriavzevii</i> could have a positive effect on the artificially controlled production of <i>baijiu</i>. In this study, an efficient acetic-acid-tolerant <i>P. kudriavzevii</i> strain, Y2, was isolated from the yellow water of strong-flavored <i>baijiu</i> brewing waste, and its molecular mechanism of acetic acid tolerance was investigated through a comparative transcriptomic analysis. The strain Y2 could tolerate 12 g/L of acetic acid. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, differentially expressed genes (DEGs) were mainly enriched in oxidative phosphorylation, the citrate cycle, glycolysis/gluconeogenesis, and carbon metabolism under low (AL group) and high (AH group) concentrations of acetic acid. However, the DEG enrichment was more profound in the AH group when compared to the control. Compared with the AL group, the expression of genes related to oxidative phosphorylation was more significantly upregulated, while in terms of the TCA cycle, phosphoenolpyruvate carboxykinase was significantly upregulated in both the AH and AL groups and was positively correlated with tolerance to acetic acid. This was followed by citrate synthase, isocitrate dehydrogenase, malate dehydrogenase, and succinate dehydrogenase. These results illustrated a possible mechanism of acid tolerance by regulating the metabolism-related pathways in <i>P. kudriavzevii</i> and provided a basis for the further investigation of the acid tolerance mechanism.https://www.mdpi.com/2311-5637/9/6/540<i>Pichia kudriavzevii</i> Y2transcriptomeacetic acidacid tolerance mechanism |
| spellingShingle | Ning Wang Puyu Zhang Xiaoli Zhou Jia Zheng Yi Ma Chenguang Liu Tao Wu Hong Li Xiaoqin Wang Hong Wang Xudong Zhao Muhammad Aamer Mehmood Hui Zhu Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance Mechanism Fermentation <i>Pichia kudriavzevii</i> Y2 transcriptome acetic acid acid tolerance mechanism |
| title | Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance Mechanism |
| title_full | Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance Mechanism |
| title_fullStr | Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance Mechanism |
| title_full_unstemmed | Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance Mechanism |
| title_short | Isolation, Identification, and Characterization of an Acid-Tolerant <i>Pichia kudriavzevii</i> and Exploration of Its Acetic Acid Tolerance Mechanism |
| title_sort | isolation identification and characterization of an acid tolerant i pichia kudriavzevii i and exploration of its acetic acid tolerance mechanism |
| topic | <i>Pichia kudriavzevii</i> Y2 transcriptome acetic acid acid tolerance mechanism |
| url | https://www.mdpi.com/2311-5637/9/6/540 |
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