High-throughput mapping of RNA solvent accessibility at the single-nucleotide resolution by RtcB ligation between a fixed 5′-OH-end linker and unique 3′-P-end fragments from hydroxyl radical cleavage

High-throughput mapping of RNA solvent accessibility at the single-nucleotide resolution by RtcB ligation between a fixed 5′-OH-end linker and unique 3′-P-end fragments from hydroxyl radical cleavage

Given the challenges for the experimental determination of RNA tertiary structures, probing solvent accessibility has become increasingly important to gain functional insights. Among various chemical probes developed, backbone-cleaving hydroxyl radical is the only one that can provide unbiased detec...

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Bibliographic Details
Main Authors: Md Solayman, Thomas Litfin, Yaoqi Zhou, Jian Zhan
Format: Article
Language:English
Published: Taylor & Francis Group 2022-12-01
Series:RNA Biology
Subjects:
sasa
hydroxyl radical
probing
rl-seq
solvent accessibility
Online Access:http://dx.doi.org/10.1080/15476286.2022.2145098

Internet

http://dx.doi.org/10.1080/15476286.2022.2145098

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