The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin
Abstract Vitamin E is classified into tocopherol (Toc) and tocotrienol (T3) based on its side chains. T3 generally has higher cellular uptake than Toc, though the responsible mechanism remains unclear. To elucidate this mechanism, we hypothesized and investigated whether serum albumin is a factor th...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34584-z |
| _version_ | 1797832177695588352 |
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| author | Takashi Nakatomi Mayuko Itaya-Takahashi Yosuke Horikoshi Naoki Shimizu Isabella Supardi Parida Mirinthorn Jutanom Takahiro Eitsuka Yoshikazu Tanaka Jean-Marc Zingg Tatsuya Matsura Kiyotaka Nakagawa |
| author_facet | Takashi Nakatomi Mayuko Itaya-Takahashi Yosuke Horikoshi Naoki Shimizu Isabella Supardi Parida Mirinthorn Jutanom Takahiro Eitsuka Yoshikazu Tanaka Jean-Marc Zingg Tatsuya Matsura Kiyotaka Nakagawa |
| author_sort | Takashi Nakatomi |
| collection | DOAJ |
| description | Abstract Vitamin E is classified into tocopherol (Toc) and tocotrienol (T3) based on its side chains. T3 generally has higher cellular uptake than Toc, though the responsible mechanism remains unclear. To elucidate this mechanism, we hypothesized and investigated whether serum albumin is a factor that induces such a difference in the cellular uptake of Toc and T3. Adding bovine serum albumin (BSA) to serum-depleted media increased the cellular uptake of T3 and decreased that of Toc, with varying degrees among α-, β-, γ-, and δ-analogs. Such enhanced uptake of α-T3 was not observed when cells were incubated under low temperature (the uptake of α-Toc was also reduced), suggesting that Toc and T3 bind to albumin to form a complex that results in differential cellular uptake of vitamin E. Fluorescence quenching study confirmed that vitamin E certainly bound to BSA, and that T3 showed a higher affinity than Toc. Molecular docking further indicated that the differential binding energy of Toc or T3 to BSA is due to the Van der Waals interactions via their side chain. Overall, these results suggested that the affinity of Toc and T3 to albumin differs due to their side chains, causing the difference in their albumin-mediated cellular uptake. Our results give a better mechanistic insight into the physiological action of vitamin E. |
| first_indexed | 2024-04-09T14:03:37Z |
| format | Article |
| id | doaj.art-b23db5b6086346a4b49f3c07308cdc56 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T14:03:37Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b23db5b6086346a4b49f3c07308cdc562023-05-07T11:10:29ZengNature PortfolioScientific Reports2045-23222023-05-0113111110.1038/s41598-023-34584-zThe difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albuminTakashi Nakatomi0Mayuko Itaya-Takahashi1Yosuke Horikoshi2Naoki Shimizu3Isabella Supardi Parida4Mirinthorn Jutanom5Takahiro Eitsuka6Yoshikazu Tanaka7Jean-Marc Zingg8Tatsuya Matsura9Kiyotaka Nakagawa10Laboratory of Food Function Analysis, Graduate School of Agricultural Science, Tohoku UniversityLaboratory of Food Function Analysis, Graduate School of Agricultural Science, Tohoku UniversityDivision of Medical Biochemistry, Department of Pathophysiological and Therapeutic Sciences, Tottori University Faculty of MedicineLaboratory of Food Function Analysis, Graduate School of Agricultural Science, Tohoku UniversityLaboratory of Food Function Analysis, Graduate School of Agricultural Science, Tohoku UniversityLaboratory of Food Function Analysis, Graduate School of Agricultural Science, Tohoku UniversityLaboratory of Food Function Analysis, Graduate School of Agricultural Science, Tohoku UniversityApplied Biological Molecular Science, Graduate School of Life Sciences, Tohoku UniversityDepartment of Biochemistry and Molecular Biology, University of MiamiDivision of Medical Biochemistry, Department of Pathophysiological and Therapeutic Sciences, Tottori University Faculty of MedicineLaboratory of Food Function Analysis, Graduate School of Agricultural Science, Tohoku UniversityAbstract Vitamin E is classified into tocopherol (Toc) and tocotrienol (T3) based on its side chains. T3 generally has higher cellular uptake than Toc, though the responsible mechanism remains unclear. To elucidate this mechanism, we hypothesized and investigated whether serum albumin is a factor that induces such a difference in the cellular uptake of Toc and T3. Adding bovine serum albumin (BSA) to serum-depleted media increased the cellular uptake of T3 and decreased that of Toc, with varying degrees among α-, β-, γ-, and δ-analogs. Such enhanced uptake of α-T3 was not observed when cells were incubated under low temperature (the uptake of α-Toc was also reduced), suggesting that Toc and T3 bind to albumin to form a complex that results in differential cellular uptake of vitamin E. Fluorescence quenching study confirmed that vitamin E certainly bound to BSA, and that T3 showed a higher affinity than Toc. Molecular docking further indicated that the differential binding energy of Toc or T3 to BSA is due to the Van der Waals interactions via their side chain. Overall, these results suggested that the affinity of Toc and T3 to albumin differs due to their side chains, causing the difference in their albumin-mediated cellular uptake. Our results give a better mechanistic insight into the physiological action of vitamin E.https://doi.org/10.1038/s41598-023-34584-z |
| spellingShingle | Takashi Nakatomi Mayuko Itaya-Takahashi Yosuke Horikoshi Naoki Shimizu Isabella Supardi Parida Mirinthorn Jutanom Takahiro Eitsuka Yoshikazu Tanaka Jean-Marc Zingg Tatsuya Matsura Kiyotaka Nakagawa The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin Scientific Reports |
| title | The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin |
| title_full | The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin |
| title_fullStr | The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin |
| title_full_unstemmed | The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin |
| title_short | The difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin |
| title_sort | difference in the cellular uptake of tocopherol and tocotrienol is influenced by their affinities to albumin |
| url | https://doi.org/10.1038/s41598-023-34584-z |
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