Circle-seq based method for eccDNA synthesis and its application as a canonical promoter independent vector for robust microRNA overexpression

Circle-seq based method for eccDNA synthesis and its application as a canonical promoter independent vector for robust microRNA overexpression

Extrachromosomal circular DNA (eccDNA) has recently gained increasing attention due to its significant role in cancer and other pathophysiologic states. The majority of circular DNAs detected by Circle-seq are small-size eccDNAs with enigmatic functions. One major technical hurdle is to synthesize e...

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Bibliographic Details
Main Authors: Jiaying Yu, Haoran Zhang, Peng Han, Xianming Jiang, Jing Li, Bo Li, Shaohua Yang, Chunxiao He, Shuang Mao, Yonghui Dang, Xi Xiang
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Computational and Structural Biotechnology Journal
Subjects:
EccDNA
EccDNA function
CAES
Artificial eccDNA
DdPCR
Circle-seq
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037023004919

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http://www.sciencedirect.com/science/article/pii/S2001037023004919

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