3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei

3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei

Summary: This protocol describes how to culture, image, and determine the nuclear position of a fluorescently tagged DNA locus in the 3D nucleoplasm of fixed Saccharomyces cerevisiae cells. Here, we propose a manual scoring method based on widefield images and an automated method based on 3D-SIM ima...

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Bibliographic Details
Main Authors: Mégane Da Mota, Julien Cau, Julio Mateos-Langerak, Armelle Lengronne, Philippe Pasero, Jérôme Poli
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell culture
Single Cell
Genetics
Microscopy
Model Organisms
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672100232X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672100232X

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