3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei

3D positioning of tagged DNA loci by widefield and super-resolution fluorescence imaging of fixed yeast nuclei

Summary: This protocol describes how to culture, image, and determine the nuclear position of a fluorescently tagged DNA locus in the 3D nucleoplasm of fixed Saccharomyces cerevisiae cells. Here, we propose a manual scoring method based on widefield images and an automated method based on 3D-SIM ima...

Full description

Bibliographic Details
Main Authors:	Mégane Da Mota, Julien Cau, Julio Mateos-Langerak, Armelle Lengronne, Philippe Pasero, Jérôme Poli
Format:	Article
Language:	English
Published:	Elsevier 2021-06-01
Series:	STAR Protocols
Subjects:	Cell culture Single Cell Genetics Microscopy Model Organisms Gene Expression
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672100232X

Similar Items

Impact of R-loops on oncogene-induced replication stress in cancer cells
by: Heuzé, Jonathan, et al.
Published: (2023-09-01)

The replication timing program in the hands of two HDACs
by: Kazumasa Yoshida, et al.
Published: (2014-07-01)

A method for labeling proteins with tags at the native genomic loci in budding yeast.
by: Qian Wang, et al.
Published: (2017-01-01)

Widefield microwave imaging in alkali vapor cells with sub-100 μm resolution
by: Andrew Horsley, et al.
Published: (2015-01-01)

Widefield and Ultra-Widefield Retinal Imaging: A Geometrical Analysis
by: Amedeo Lucente, et al.
Published: (2023-01-01)

Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling
by: Katie L. Whytock, et al.
Published: (2023-03-01)

Heavy nuclei, super heavy nuclei and neutron starts
by: 380093 Irvine, J. M. (John Maxwell)

Nuclei isolation from surgically resected human hippocampus
by: Fatma Ayhan, et al.
Published: (2021-12-01)

Extraction and sequencing of single nuclei from murine skeletal muscles
by: Matthieu Dos Santos, et al.
Published: (2021-09-01)

Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing
by: Tamer Butto, et al.
Published: (2023-03-01)

Protocol for the isolation and single-nuclei multiomic analyses of the human fetal epicardium
by: Stanislao Igor Travisano, et al.
Published: (2024-06-01)

Widefield Parametric Polar Format Algorithm for Spotlight SAR Imaging
by: Zhichao Meng, et al.
Published: (2023-01-01)

The Role of Widefield and Ultra Widefield Optical Coherence Tomography in the Diagnosis and Management of Vitreoretinal Diseases
by: Matteo Ripa, et al.
Published: (2022-09-01)

Fast widefield scan provides tunable and uniform illumination optimizing super-resolution microscopy on large fields
by: Adrien Mau, et al.
Published: (2021-05-01)

The Murchison Widefield Array Correlator
by: Cappallo, Roger J., et al.
Published: (2015)

Science with the Murchison Widefield Array
by: Cappallo, Roger J., et al.
Published: (2014)

Electrical monopole transitions in non-spherical nuclei/
by: Davydov, A. S. (Aleksandr Sergeevich), 1912-, et al.

Proliferative Sickle Cell Retinopathy in the Retinal Periphery Detected by Ultra-Widefield Imaging: A Case Report
by: Audrey Giocanti-Aurégan, et al.
Published: (2023-04-01)

An investigation of the nuclei of hülle cells of Aspergillus nidulans
by: Marcos D.F. Carvalho, et al.
Published: (2002-01-01)

Thermal instability of cell nuclei
by: Enrico Warmt, et al.
Published: (2014-01-01)

Cellular loci involved in the development of brain arteriovenous malformations
by: Zahra Shabani, et al.
Published: (2022-09-01)

Cell division- and DNA replication-free reprogramming of somatic nuclei for embryonic transcription
by: Junko Tomikawa, et al.
Published: (2021-11-01)

HaloTag-based reporters for sparse labeling and cell tracking
by: Lydie Couturier, et al.
Published: (2022-12-01)

The Murchison Widefield Array: Design Overview
by: Lonsdale, Colin John, et al.
Published: (2010)

Sources of widefield fluorescence from the brain
by: Jack Waters
Published: (2020-11-01)

Ultra-widefield imaging in Takayasu arteritis
by: Manmath K Das, et al.
Published: (2022-01-01)

Widefield imaging of retinal and choroidal tumors
by: Natalia F. Callaway, et al.
Published: (2019-12-01)

Tissue-specific tagging of endogenous loci in Drosophila melanogaster
by: Kate Koles, et al.
Published: (2016-01-01)

Cervical cell nuclei segmentation based on GC-UNet
by: Enguang Zhang, et al.
Published: (2023-07-01)

Systematic Comparison of Cell Wall-Related Proteins of Different Yeasts
by: Mateja Lozančić, et al.
Published: (2021-02-01)

Mitochondria in the Nuclei of Rat Myocardial Cells
by: Chupalav M. Eldarov, et al.
Published: (2020-03-01)

Projections from the dorsal and ventral cochlear nuclei to the medial geniculate body
by: Brett R Schofield, et al.
Published: (2014-03-01)

Chemical composition of cell walls and membranes of yeasts /
by: 289725 Nickerson, Walter J.

Vestibular Nuclei: A New Neural Stem Cell Niche?
by: Guillaume Rastoldo, et al.
Published: (2022-11-01)

Flower-Like Plasma Cell Nuclei in Multiple Myeloma
by: Abibatou Sall, et al.
Published: (2021-06-01)

Tagging transferrin receptor with a disulfide FRET probe to gauge the redox state in endosomal compartments
by: Bi, Xiaobao, et al.
Published: (2021)

Aggregate Filamentous Growth Responses in Yeast
by: Jacky Chow, et al.
Published: (2019-04-01)

Fabrication of super capacitor and perovskite-sensitized solar cell for the assembly of photo-super capacitor
by: Ng, Chi Huey
Published: (2018)

Metabolic pathways further increase the complexity of cell size control in budding yeast
by: Jorrit M. Enserink
Published: (2014-08-01)

Single-nuclei characterization of pervasive transcriptional signatures across organs in response to COVID-19
by: The COVID Tissue Atlas Consortium, et al.
Published: (2023-10-01)