The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease models
Abstract Baboon models are often used to investigate haemostatic diseases, such as acquired thrombotic thrombocytopenic purpura or bacterial sepsis-induced disseminated intravascular coagulation, and their potential treatment with novel drugs. Thrombin generation is vital for these models, and an im...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-50341-8 |
| _version_ | 1797371164509601792 |
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| author | J. Joubert S. M. Meiring W. J. Janse van Rensburg |
| author_facet | J. Joubert S. M. Meiring W. J. Janse van Rensburg |
| author_sort | J. Joubert |
| collection | DOAJ |
| description | Abstract Baboon models are often used to investigate haemostatic diseases, such as acquired thrombotic thrombocytopenic purpura or bacterial sepsis-induced disseminated intravascular coagulation, and their potential treatment with novel drugs. Thrombin generation is vital for these models, and an important potential therapeutic target. We investigated the thrombin generation profile of the Chacma baboon (Papio ursinus – a common pre-clinical model) including the effects of sex and ABO blood group. Thrombin generation curves, lag times, peak heights, times-to-peak, velocity indexes and Endogenous Thrombin Potentials (ETPs) of 40 adult Chacma baboons were assessed and compared with normal human plasma, using a low concentration of tissue factor (1 pM) and phospholipids. Reference intervals were calculated, and results compared between O and non-O ABO blood groups, and between males and females. Lag times of all baboons fell within the human reference interval. Most animals (n = 32; 80%) had times-to-peak above, and velocity indexes and peak heights markedly below (n = 27; 68%) the human range. However, 97.5% of baboons had an ETP above the human reference interval, indicating greater overall thrombin generation. ABO blood group had no effect, but males (n = 14; 35%) had less potent thrombin generation than females (n = 26; 65%), with significantly longer lag times (p = 0.0475), lower peak thrombin concentrations (p = 0.0203), and lower ETPs (p = 0.0238). Chacma baboons have greater overall endogenous thrombin generation potentials than humans, which is even more prominent in females. This should be considered when designing future baboon model experiments involving the haemostatic system, or when evaluating novel therapies in these animals. |
| first_indexed | 2024-03-08T18:15:52Z |
| format | Article |
| id | doaj.art-b2c07185ee7e4c79b8c09f5c7f248a33 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T18:15:52Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b2c07185ee7e4c79b8c09f5c7f248a332023-12-31T12:10:37ZengNature PortfolioScientific Reports2045-23222023-12-0113111010.1038/s41598-023-50341-8The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease modelsJ. Joubert0S. M. Meiring1W. J. Janse van Rensburg2Department of Haematology and Cell Biology, School of Pathology, Faculty of Health Sciences, University of the Free StateDepartment of Haematology and Cell Biology, School of Pathology, Faculty of Health Sciences, University of the Free StateHuman Molecular Biology Unit, School of Biomedical Sciences, Faculty of Health Sciences, University of the Free StateAbstract Baboon models are often used to investigate haemostatic diseases, such as acquired thrombotic thrombocytopenic purpura or bacterial sepsis-induced disseminated intravascular coagulation, and their potential treatment with novel drugs. Thrombin generation is vital for these models, and an important potential therapeutic target. We investigated the thrombin generation profile of the Chacma baboon (Papio ursinus – a common pre-clinical model) including the effects of sex and ABO blood group. Thrombin generation curves, lag times, peak heights, times-to-peak, velocity indexes and Endogenous Thrombin Potentials (ETPs) of 40 adult Chacma baboons were assessed and compared with normal human plasma, using a low concentration of tissue factor (1 pM) and phospholipids. Reference intervals were calculated, and results compared between O and non-O ABO blood groups, and between males and females. Lag times of all baboons fell within the human reference interval. Most animals (n = 32; 80%) had times-to-peak above, and velocity indexes and peak heights markedly below (n = 27; 68%) the human range. However, 97.5% of baboons had an ETP above the human reference interval, indicating greater overall thrombin generation. ABO blood group had no effect, but males (n = 14; 35%) had less potent thrombin generation than females (n = 26; 65%), with significantly longer lag times (p = 0.0475), lower peak thrombin concentrations (p = 0.0203), and lower ETPs (p = 0.0238). Chacma baboons have greater overall endogenous thrombin generation potentials than humans, which is even more prominent in females. This should be considered when designing future baboon model experiments involving the haemostatic system, or when evaluating novel therapies in these animals.https://doi.org/10.1038/s41598-023-50341-8 |
| spellingShingle | J. Joubert S. M. Meiring W. J. Janse van Rensburg The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease models Scientific Reports |
| title | The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease models |
| title_full | The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease models |
| title_fullStr | The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease models |
| title_full_unstemmed | The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease models |
| title_short | The thrombin generation capability of the Chacma baboon (Papio ursinus): implications for haemostatic disease models |
| title_sort | thrombin generation capability of the chacma baboon papio ursinus implications for haemostatic disease models |
| url | https://doi.org/10.1038/s41598-023-50341-8 |
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