Development of real-time reverse transcription PCR for detection of Maize chlorotic mottle virus based on a novel molecular marker
Maize chlorotic mottle virus (MCMV) infects maize plants and causes significant losses in corn production worldwide. In this study, a real-time TaqMan RT-PCR assay for efficient detection of MCMV was described. A pair of primers amplifying a 131-bp DNA fragment and a TaqMan probe was designed target...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2016-12-01
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Series: | Cogent Food & Agriculture |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/23311932.2016.1224047 |