Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR

Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR

The aim of the study was to identify the Pm2, Pm3a, Pm4b and Pm6 genes and to develop multiplex PCR reaction conditions to reduce time and limit analysis costs. The following molecular markers were used for gene identification: Xcfd81, Whs350 and Xgwm205 (for Pm2), Pm3a (for Pm3a), STS_241 and Xgwm3...

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Bibliographic Details
Main Authors: Tomkowiak Agnieszka, Skowrońska Roksana, Weigt Dorota, Kwiatek Michał, Nawracała Jerzy, Kowalczewski Przemysław Łukasz, Pluta Mateusz
Format: Article
Language:English
Published: De Gruyter 2019-03-01
Series:Open Chemistry
Subjects:
wheat
powdery mildew
ssr molecular markers
multiplex pcr
Online Access:http://www.degruyter.com/view/j/chem.2019.17.issue-1/chem-2019-0024/chem-2019-0024.xml?format=INT

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http://www.degruyter.com/view/j/chem.2019.17.issue-1/chem-2019-0024/chem-2019-0024.xml?format=INT

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