Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detector
In this paper, a small dual light-emitting diode (LED)-based detector for FIA process analyser has been designed. The detector’s optical parts comprise a flow-through cell, a dual-blue LED and a photodiode. Neither mirrors nor lenses are used. The optical path for the first LED detects the blank, wh...
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| Format: | Article |
| Language: | English |
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Maejo University
2008-02-01
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| Series: | Maejo International Journal of Science and Technology |
| Subjects: | |
| Online Access: | http://www.mijst.mju.ac.th/vol2/201-209.pdf |
| _version_ | 1811269404344713216 |
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| author | Prinya Masawat |
| author_facet | Prinya Masawat |
| author_sort | Prinya Masawat |
| collection | DOAJ |
| description | In this paper, a small dual light-emitting diode (LED)-based detector for FIA process analyser has been designed. The detector’s optical parts comprise a flow-through cell, a dual-blue LED and a photodiode. Neither mirrors nor lenses are used. The optical path for the first LED detects the blank, while the other LED detects the sample. The detector’s electronic components including a signal amplifier and an A/D converter are integrated on one small board connected to a PC for measuring the results. The designed spectrophotometric detector was used for the determination of tetracycline antibiotics. Uranyl acetate was used as a reagent forming orange-red complexes with the drugs in N,N– dimethylformamide. The complexes show absorption maxima at 410, 416 and 408 nm for tetracycline hydrochloride (TCH), chlortetracycline hydrochloride (CTCH), and doxycycline hydrochloride (DCH), respectively. The detection limit was found to be 0.38, 0.75, 1.44 µg mL-1 and the linear range was obtained at 1.0-3.0, 3.0-5.0, and 3.0-10.0 µg mL-1 for TCH, CTCH and DCH, respectively. The proposed method has been successfully applied to the determination of tetracycline antibiotic residues in milk samples. Moreover, this method is an environmentally friendly approach and suitable for routine analysis. |
| first_indexed | 2024-04-12T21:41:26Z |
| format | Article |
| id | doaj.art-b30e70bd6d734d9bbabbce4129134207 |
| institution | Directory Open Access Journal |
| issn | 1905-7873 |
| language | English |
| last_indexed | 2024-04-12T21:41:26Z |
| publishDate | 2008-02-01 |
| publisher | Maejo University |
| record_format | Article |
| series | Maejo International Journal of Science and Technology |
| spelling | doaj.art-b30e70bd6d734d9bbabbce41291342072022-12-22T03:15:45ZengMaejo UniversityMaejo International Journal of Science and Technology1905-78732008-02-01201201209Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detectorPrinya MasawatIn this paper, a small dual light-emitting diode (LED)-based detector for FIA process analyser has been designed. The detector’s optical parts comprise a flow-through cell, a dual-blue LED and a photodiode. Neither mirrors nor lenses are used. The optical path for the first LED detects the blank, while the other LED detects the sample. The detector’s electronic components including a signal amplifier and an A/D converter are integrated on one small board connected to a PC for measuring the results. The designed spectrophotometric detector was used for the determination of tetracycline antibiotics. Uranyl acetate was used as a reagent forming orange-red complexes with the drugs in N,N– dimethylformamide. The complexes show absorption maxima at 410, 416 and 408 nm for tetracycline hydrochloride (TCH), chlortetracycline hydrochloride (CTCH), and doxycycline hydrochloride (DCH), respectively. The detection limit was found to be 0.38, 0.75, 1.44 µg mL-1 and the linear range was obtained at 1.0-3.0, 3.0-5.0, and 3.0-10.0 µg mL-1 for TCH, CTCH and DCH, respectively. The proposed method has been successfully applied to the determination of tetracycline antibiotic residues in milk samples. Moreover, this method is an environmentally friendly approach and suitable for routine analysis.http://www.mijst.mju.ac.th/vol2/201-209.pdftetracyclinechlortetracyclinedoxycyclineflow injectionlight-emitting diodeuranyl acetate |
| spellingShingle | Prinya Masawat Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detector Maejo International Journal of Science and Technology tetracycline chlortetracycline doxycycline flow injection light-emitting diode uranyl acetate |
| title | Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detector |
| title_full | Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detector |
| title_fullStr | Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detector |
| title_full_unstemmed | Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detector |
| title_short | Spectrophotometric flow-injection analysis assay of tetracycline antibiotics using a dual light-emitting diode based detector |
| title_sort | spectrophotometric flow injection analysis assay of tetracycline antibiotics using a dual light emitting diode based detector |
| topic | tetracycline chlortetracycline doxycycline flow injection light-emitting diode uranyl acetate |
| url | http://www.mijst.mju.ac.th/vol2/201-209.pdf |
| work_keys_str_mv | AT prinyamasawat spectrophotometricflowinjectionanalysisassayoftetracyclineantibioticsusingaduallightemittingdiodebaseddetector |