In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups
The in vitro effects of four nutraceuticals, catechin hydrate, gallic acid, α-tocopherol and ascorbic acid, on the ability of human osteoarthritic chondrocytes of two female obese groups to form articular cartilage (AC) tissues and to reduce inflammation were investigated. Group 1 represented thirte...
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2021-01-01
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| Series: | Journal of Nutritional Science |
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| Online Access: | https://www.cambridge.org/core/product/identifier/S2048679021000793/type/journal_article |
| _version_ | 1811155935192678400 |
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| author | Mahmoud Amr Alia Mallah Haneen Abusharkh Bernard Van Wie Arda Gozen Juana Mendenhall Vincent Idone Edwin Tingstad Nehal I. Abu-Lail |
| author_facet | Mahmoud Amr Alia Mallah Haneen Abusharkh Bernard Van Wie Arda Gozen Juana Mendenhall Vincent Idone Edwin Tingstad Nehal I. Abu-Lail |
| author_sort | Mahmoud Amr |
| collection | DOAJ |
| description | The in vitro effects of four nutraceuticals, catechin hydrate, gallic acid, α-tocopherol and ascorbic acid, on the ability of human osteoarthritic chondrocytes of two female obese groups to form articular cartilage (AC) tissues and to reduce inflammation were investigated. Group 1 represented thirteen females in the 50–69 years old range, an average weight of 100 kg and an average body mass index (BMI) of 34⋅06 kg/m2. Group 2 was constituted of three females in the 70–80 years old range, an average weight of 75 kg and an average BMI of 31⋅43 kg/m2. The efficacy of nutraceuticals was assessed in monolayer cultures using histological, colorimetric and mRNA gene expression analyses. AC engineered tissues of group 1 produced less total collagen and COL2A1 (38-fold), and higher COL10A1 (2⋅7-fold), MMP13 (50-fold) and NOS2 (15-fold) mRNA levels than those of group 2. In comparison, engineered tissues of group 1 had a significant decrease in NO levels from day 1 to day 21 (2⋅6-fold), as well as higher mRNA levels of FOXO1 (2-fold) and TNFAIP6 (16-fold) compared to group 2. Catechin hydrate decreased NO levels significantly in group 1 (1⋅5-fold) while increasing NO levels significantly in group 2 (3⋅8-fold). No differences from the negative control were observed in the presence of other nutraceuticals for either group. In conclusion, engineered tissues of the younger but heavier patients responded better to nutraceuticals than those from the older but leaner study participants. Finally, cells of group 2 formed better AC tissues with less inflammation and better extracellular matrix than cells of group 1. |
| first_indexed | 2024-04-10T04:43:15Z |
| format | Article |
| id | doaj.art-b325a1e7ab034bb1b4c4a5719fd822b5 |
| institution | Directory Open Access Journal |
| issn | 2048-6790 |
| language | English |
| last_indexed | 2024-04-10T04:43:15Z |
| publishDate | 2021-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Nutritional Science |
| spelling | doaj.art-b325a1e7ab034bb1b4c4a5719fd822b52023-03-09T12:38:45ZengCambridge University PressJournal of Nutritional Science2048-67902021-01-011010.1017/jns.2021.79In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groupsMahmoud Amr0Alia Mallah1Haneen Abusharkh2Bernard Van Wie3Arda Gozen4Juana Mendenhall5Vincent Idone6Edwin Tingstad7Nehal I. Abu-Lail8https://orcid.org/0000-0002-2319-2450Department of Biomedical Engineering and Chemical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USADepartment of Biomedical Engineering and Chemical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USAGene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-6515, USAGene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-6515, USASchool of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USADepartment of Chemistry, Morehouse College, Atlanta, GA 30314, USARegeneron Pharmaceuticals Inc, Tarrytown, NY 10591, USAInland Orthopedic Surgery and Sports Clinic, Pullman, WA 99163, USADepartment of Biomedical Engineering and Chemical Engineering, The University of Texas at San Antonio, San Antonio, TX 78249, USAThe in vitro effects of four nutraceuticals, catechin hydrate, gallic acid, α-tocopherol and ascorbic acid, on the ability of human osteoarthritic chondrocytes of two female obese groups to form articular cartilage (AC) tissues and to reduce inflammation were investigated. Group 1 represented thirteen females in the 50–69 years old range, an average weight of 100 kg and an average body mass index (BMI) of 34⋅06 kg/m2. Group 2 was constituted of three females in the 70–80 years old range, an average weight of 75 kg and an average BMI of 31⋅43 kg/m2. The efficacy of nutraceuticals was assessed in monolayer cultures using histological, colorimetric and mRNA gene expression analyses. AC engineered tissues of group 1 produced less total collagen and COL2A1 (38-fold), and higher COL10A1 (2⋅7-fold), MMP13 (50-fold) and NOS2 (15-fold) mRNA levels than those of group 2. In comparison, engineered tissues of group 1 had a significant decrease in NO levels from day 1 to day 21 (2⋅6-fold), as well as higher mRNA levels of FOXO1 (2-fold) and TNFAIP6 (16-fold) compared to group 2. Catechin hydrate decreased NO levels significantly in group 1 (1⋅5-fold) while increasing NO levels significantly in group 2 (3⋅8-fold). No differences from the negative control were observed in the presence of other nutraceuticals for either group. In conclusion, engineered tissues of the younger but heavier patients responded better to nutraceuticals than those from the older but leaner study participants. Finally, cells of group 2 formed better AC tissues with less inflammation and better extracellular matrix than cells of group 1.https://www.cambridge.org/core/product/identifier/S2048679021000793/type/journal_articleAgeArticualr cartilageCatechin hydrateNutraceuticalsOsteoarthritisWeight |
| spellingShingle | Mahmoud Amr Alia Mallah Haneen Abusharkh Bernard Van Wie Arda Gozen Juana Mendenhall Vincent Idone Edwin Tingstad Nehal I. Abu-Lail In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups Journal of Nutritional Science Age Articualr cartilage Catechin hydrate Nutraceuticals Osteoarthritis Weight |
| title | In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups |
| title_full | In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups |
| title_fullStr | In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups |
| title_full_unstemmed | In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups |
| title_short | In vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups |
| title_sort | in vitro effects of nutraceutical treatment on human osteoarthritic chondrocytes of females of different age and weight groups |
| topic | Age Articualr cartilage Catechin hydrate Nutraceuticals Osteoarthritis Weight |
| url | https://www.cambridge.org/core/product/identifier/S2048679021000793/type/journal_article |
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