Synthetic chimeric nucleases function for efficient genome editing

Synthetic chimeric nucleases function for efficient genome editing

CRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and specificities.

Bibliographic Details
Main Authors: R. M. Liu, L. L. Liang, E. Freed, H. Chang, E. Oh, Z. Y. Liu, A. Garst, C. A. Eckert, R. T. Gill
Format: Article
Language:English
Published: Nature Portfolio 2019-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-13500-y

Internet

https://doi.org/10.1038/s41467-019-13500-y

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