Synthetic chimeric nucleases function for efficient genome editing
CRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and specificities.
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-13500-y |
| _version_ | 1818683312674177024 |
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| author | R. M. Liu L. L. Liang E. Freed H. Chang E. Oh Z. Y. Liu A. Garst C. A. Eckert R. T. Gill |
| author_facet | R. M. Liu L. L. Liang E. Freed H. Chang E. Oh Z. Y. Liu A. Garst C. A. Eckert R. T. Gill |
| author_sort | R. M. Liu |
| collection | DOAJ |
| description | CRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and specificities. |
| first_indexed | 2024-12-17T10:32:44Z |
| format | Article |
| id | doaj.art-b3426a5c4a194f89b3f9de6d909729e5 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T10:32:44Z |
| publishDate | 2019-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-b3426a5c4a194f89b3f9de6d909729e52022-12-21T21:52:28ZengNature PortfolioNature Communications2041-17232019-12-0110111110.1038/s41467-019-13500-ySynthetic chimeric nucleases function for efficient genome editingR. M. Liu0L. L. Liang1E. Freed2H. Chang3E. Oh4Z. Y. Liu5A. Garst6C. A. Eckert7R. T. Gill8Renewable and Sustainable Energy Institute (RASEI), University of ColoradoRenewable and Sustainable Energy Institute (RASEI), University of ColoradoRenewable and Sustainable Energy Institute (RASEI), University of ColoradoDepartment of Biochemistry, University of ColoradoRenewable and Sustainable Energy Institute (RASEI), University of ColoradoDepartment of Biochemistry, University of ColoradoInscripta, Inc.Renewable and Sustainable Energy Institute (RASEI), University of ColoradoRenewable and Sustainable Energy Institute (RASEI), University of ColoradoCRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and specificities.https://doi.org/10.1038/s41467-019-13500-y |
| spellingShingle | R. M. Liu L. L. Liang E. Freed H. Chang E. Oh Z. Y. Liu A. Garst C. A. Eckert R. T. Gill Synthetic chimeric nucleases function for efficient genome editing Nature Communications |
| title | Synthetic chimeric nucleases function for efficient genome editing |
| title_full | Synthetic chimeric nucleases function for efficient genome editing |
| title_fullStr | Synthetic chimeric nucleases function for efficient genome editing |
| title_full_unstemmed | Synthetic chimeric nucleases function for efficient genome editing |
| title_short | Synthetic chimeric nucleases function for efficient genome editing |
| title_sort | synthetic chimeric nucleases function for efficient genome editing |
| url | https://doi.org/10.1038/s41467-019-13500-y |
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