A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice.
Dietary plant sterols (PS) reduce serum total and LDL-cholesterol in hyperlipidemic animal models and in humans. This hypocholesterolemic effect is generally ascribed to inhibition of cholesterol absorption. However, whether this effect fully explains the reported strong induction of neutral sterol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3128081?pdf=render |
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| author | Gemma Brufau Folkert Kuipers Yuguang Lin Elke A Trautwein Albert K Groen |
| author_facet | Gemma Brufau Folkert Kuipers Yuguang Lin Elke A Trautwein Albert K Groen |
| author_sort | Gemma Brufau |
| collection | DOAJ |
| description | Dietary plant sterols (PS) reduce serum total and LDL-cholesterol in hyperlipidemic animal models and in humans. This hypocholesterolemic effect is generally ascribed to inhibition of cholesterol absorption. However, whether this effect fully explains the reported strong induction of neutral sterol excretion upon plant sterol feeding is not known. Recent data demonstrate that the intestine directly mediates plasma cholesterol excretion into feces, i.e., without involvement of the hepato-biliary route.Aim of this study was to determine whether stimulation of fecal neutral sterol loss during PS feeding is (partly) explained by increased intestinal cholesterol excretion and to assess the role of the cholesterol transporter Abcg5/Abcg8 herein.Wild-type mice were fed a control diet or diets enriched with increasing amounts of PS (1%, 2%, 4% or 8%, wt/wt) for two weeks. In addition, Abcg5(-/-) mice were fed either control or 8% PS diet. PS feeding resulted in a dose-dependent decrease of fractional cholesterol absorption (∼2-7-fold reduction) in wild-type mice and ∼80% reduction in Abcg5(-/-) mice. Furthermore, PS feeding led to a strong, dose-independent induction of neutral sterol excretion (3.4-fold in wild-types and 2.7-fold in Abcg5(-/-) mice) without changes in biliary cholesterol secretion. It was calculated that PS feeding stimulated intestinal cholesterol excretion by ∼500% in wild-type mice and by ∼250% in Abcg5(-/-).Our data indicate that in mice the cholesterol-lowering effects of PS are to a large extent attributable to stimulation of intestinal, non-bile derived, cholesterol excretion. The Abcg5/Abcg8 heterodimer is involved in facilitating this PS-induced flux of cholesterol. |
| first_indexed | 2024-12-11T23:24:58Z |
| format | Article |
| id | doaj.art-b35fdd954f094608b723648dd0a1e040 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T23:24:58Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b35fdd954f094608b723648dd0a1e0402022-12-22T00:46:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0166e2157610.1371/journal.pone.0021576A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice.Gemma BrufauFolkert KuipersYuguang LinElke A TrautweinAlbert K GroenDietary plant sterols (PS) reduce serum total and LDL-cholesterol in hyperlipidemic animal models and in humans. This hypocholesterolemic effect is generally ascribed to inhibition of cholesterol absorption. However, whether this effect fully explains the reported strong induction of neutral sterol excretion upon plant sterol feeding is not known. Recent data demonstrate that the intestine directly mediates plasma cholesterol excretion into feces, i.e., without involvement of the hepato-biliary route.Aim of this study was to determine whether stimulation of fecal neutral sterol loss during PS feeding is (partly) explained by increased intestinal cholesterol excretion and to assess the role of the cholesterol transporter Abcg5/Abcg8 herein.Wild-type mice were fed a control diet or diets enriched with increasing amounts of PS (1%, 2%, 4% or 8%, wt/wt) for two weeks. In addition, Abcg5(-/-) mice were fed either control or 8% PS diet. PS feeding resulted in a dose-dependent decrease of fractional cholesterol absorption (∼2-7-fold reduction) in wild-type mice and ∼80% reduction in Abcg5(-/-) mice. Furthermore, PS feeding led to a strong, dose-independent induction of neutral sterol excretion (3.4-fold in wild-types and 2.7-fold in Abcg5(-/-) mice) without changes in biliary cholesterol secretion. It was calculated that PS feeding stimulated intestinal cholesterol excretion by ∼500% in wild-type mice and by ∼250% in Abcg5(-/-).Our data indicate that in mice the cholesterol-lowering effects of PS are to a large extent attributable to stimulation of intestinal, non-bile derived, cholesterol excretion. The Abcg5/Abcg8 heterodimer is involved in facilitating this PS-induced flux of cholesterol.http://europepmc.org/articles/PMC3128081?pdf=render |
| spellingShingle | Gemma Brufau Folkert Kuipers Yuguang Lin Elke A Trautwein Albert K Groen A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice. PLoS ONE |
| title | A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice. |
| title_full | A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice. |
| title_fullStr | A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice. |
| title_full_unstemmed | A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice. |
| title_short | A reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice. |
| title_sort | reappraisal of the mechanism by which plant sterols promote neutral sterol loss in mice |
| url | http://europepmc.org/articles/PMC3128081?pdf=render |
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