An artificial triazole backbone linkage provides a split-and-click strategy to bioactive chemically modified CRISPR sgRNA
For CRISPR-Cas9 genome editing, Cas9 protein is guided to its target by single guide (sg) RNA. Here, the authors synthesised sgRNAs via convergent ‘click’ ligation of variable 20-mer RNAs that target the genome and a Cas9-binding 79-mer chimeric RNA/2´-OMe RNA of fixed sequence in a single tube....
| मुख्य लेखकों: | , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
Nature Portfolio
2019-04-01
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| श्रृंखला: | Nature Communications |
| ऑनलाइन पहुंच: | https://doi.org/10.1038/s41467-019-09600-4 |