Improved <i>piggyBac</i> Transformation with Capped Transposase mRNA in Pest Insects

Improved <i>piggyBac</i> Transformation with Capped Transposase mRNA in Pest Insects

Creating transgenic insects is a key technology in insect genetics and molecular biology. A widely used instrument in insect transgenesis is the <i>piggyBac</i> transposase, resulting in essentially random genomic integrations. In contrast, site-specific recombinases allow the targeted i...

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Bibliographic Details
Main Authors: Irina Häcker, Tanja Rehling, Henrik Schlosser, Daniela Mayorga-Ch, Mara Heilig, Ying Yan, Peter A. Armbruster, Marc F. Schetelig
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:International Journal of Molecular Sciences
Subjects:
insect transgenesis
<i>Aedes</i>
<i>Drosophila suzukii</i>
tephritids
transformation efficiency
recombination efficiency
Online Access:https://www.mdpi.com/1422-0067/24/20/15155

Internet

https://www.mdpi.com/1422-0067/24/20/15155

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