Complementation Studies of Bacteriophage λ O Amber Mutants by Allelic Forms of O Expressed from Plasmid, and O-P Interaction Phenotypes

Complementation Studies of Bacteriophage λ O Amber Mutants by Allelic Forms of O Expressed from Plasmid, and O-P Interaction Phenotypes

λ genes O and P are required for replication initiation from the bacteriophage λ origin site, oriλ, located within gene O. Questions have persisted for years about whether O-defects can indeed be complemented in trans. We show the effect of original null mutations in O and the influence of four orig...

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Bibliographic Details
Main Authors: Sidney Hayes, Karthic Rajamanickam, Connie Hayes
Format: Article
Language:English
Published: MDPI AG 2018-04-01
Series:Antibiotics
Subjects:
bacteriophage lambda (λ)
bi-directional replication initiation from oriλ
O and P initiator proteins
oriλ interaction site
O complementation by oriλ-defective alleles
influence of O:P interactions on cell growth and O activity
Online Access:http://www.mdpi.com/2079-6382/7/2/31

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http://www.mdpi.com/2079-6382/7/2/31

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