12S gene metabarcoding with DNA standard quantifies marine bony fish environmental DNA, identifies threshold for reproducible detection, and overcomes distortion due to amplification of non‐fish DNA
Abstract Single‐species PCR assays accurately measure eDNA concentration. Here we test whether multi‐species PCR, that is, metabarcoding, with an internal standard can quantify eDNA of marine bony fish. Replicate amplifications with Riaz 12 S gene primers were spiked with known amounts of a non‐fish...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
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| Series: | Environmental DNA |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/edn3.376 |