FocAn: automated 3D analysis of DNA repair foci in image stacks acquired by confocal fluorescence microscopy

FocAn: automated 3D analysis of DNA repair foci in image stacks acquired by confocal fluorescence microscopy

Abstract Background Phosphorylated histone H2AX, also known as γH2AX, forms μm-sized nuclear foci at the sites of DNA double-strand breaks (DSBs) induced by ionizing radiation and other agents. Due to their specificity and sensitivity, γH2AX immunoassays have become the gold standard for studying DS...

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Bibliographic Details
Main Authors: Simon Memmel, Dmitri Sisario, Heiko Zimmermann, Markus Sauer, Vladimir L. Sukhorukov, Cholpon S. Djuzenova, Michael Flentje
Format: Article
Language:English
Published: BMC 2020-01-01
Series:BMC Bioinformatics
Subjects:
DNA double-strand breaks
ImageJ plugin
γH2AX-foci
Automated analysis
Ionizing radiation
Open-source tool
Online Access:https://doi.org/10.1186/s12859-020-3370-8

Internet

https://doi.org/10.1186/s12859-020-3370-8

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