Focus image scanning microscopy for sharp and gentle super-resolved microscopy
Super-resolution microscopy techniques can be challenging for live cells and thick samples. Here, the authors propose a method to reduce beam intensity and remove out-of-focus fluorescence background in image-scanning microscopy (ISM) and its combination with stimulated emission depletion (STED).
Main Authors: | Giorgio Tortarolo, Alessandro Zunino, Francesco Fersini, Marco Castello, Simonluca Piazza, Colin J. R. Sheppard, Paolo Bianchini, Alberto Diaspro, Sami Koho, Giuseppe Vicidomini |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2022-12-01
|
Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-022-35333-y |
Similar Items
-
Background Rejection in Two-Photon Fluorescence Image Scanning Microscopy
by: Colin J. R. Sheppard, et al.
Published: (2023-05-01) -
On the Advent of Super-Resolution Microscopy in the Realm of Polycomb Proteins
by: Irene Nepita, et al.
Published: (2023-02-01) -
Image Scanning Microscopy to Investigate Polycomb Protein Colocalization onto Chromatin
by: Irene Nepita, et al.
Published: (2023-01-01) -
The Development of Microscopy for Super-Resolution: Confocal Microscopy, and Image Scanning Microscopy
by: Colin J. R. Sheppard
Published: (2021-09-01) -
Pushing the performance of image scanning microscopy to its limits with maximum likelihood reconstruction
by: Garré Giacomo, et al.
Published: (2023-01-01)