Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy
Abstract PCSK9, which is closely related to atherosclerosis, is significantly expressed in vascular smooth muscle cells (VSMCs). Moreover, Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) mediated phenotypic transformation, abnormal proliferation, and migration of VSMCs play key roles in accele...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-01904-4 |
| _version_ | 1797822691522117632 |
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| author | Zhenxian Li Haimei Zhu Hao Liu Dayue Liu Jianhe Liu Jiazheng Jiang Yi Zhang Zhang Qin Yijia Xu Yuan Peng Bin Liu Yun Long |
| author_facet | Zhenxian Li Haimei Zhu Hao Liu Dayue Liu Jianhe Liu Jiazheng Jiang Yi Zhang Zhang Qin Yijia Xu Yuan Peng Bin Liu Yun Long |
| author_sort | Zhenxian Li |
| collection | DOAJ |
| description | Abstract PCSK9, which is closely related to atherosclerosis, is significantly expressed in vascular smooth muscle cells (VSMCs). Moreover, Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) mediated phenotypic transformation, abnormal proliferation, and migration of VSMCs play key roles in accelerating atherosclerosis. In this study, by utilizing the significant advantages of nano-materials, a biomimetic nanoliposome loading with Evolocumab (Evol), a PCSK9 inhibitor, was designed to alleviate atherosclerosis. In vitro results showed that (Lipo + M)@E NPs up-regulated the levels of α-SMA and Vimentin, while inhibiting the expression of OPN, which finally result in the inhibition of the phenotypic transition, excessive proliferation, and migration of VSMCs. In addition, the long circulation, excellent targeting, and accumulation performance of (Lipo + M)@E NPs significantly decreased the expression of PCSK9 in serum and VSMCs within the plaque of ApoE−/− mice. |
| first_indexed | 2024-03-13T10:12:52Z |
| format | Article |
| id | doaj.art-b400565aae64461a99cdfdbf86afbad3 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-13T10:12:52Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-b400565aae64461a99cdfdbf86afbad32023-05-21T11:26:11ZengBMCJournal of Nanobiotechnology1477-31552023-05-0121112110.1186/s12951-023-01904-4Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapyZhenxian Li0Haimei Zhu1Hao Liu2Dayue Liu3Jianhe Liu4Jiazheng Jiang5Yi Zhang6Zhang Qin7Yijia Xu8Yuan Peng9Bin Liu10Yun Long11Department of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyDepartment of Pain, The First Hospital of Hunan University of Chinese MedicineDepartment of Rehabilitation, The Second Xiangya Hospital, Central South UniversityDepartment of Physiology and Pathophysiology, NHC Key Laboratory of Metabolic Cardiovascular Diseases Research, School of Basic Medical Sciences, Ningxia Medical UniversityDepartment of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyDepartment of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyDepartment of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyDepartment of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyDepartment of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyDepartment of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyCollege of Biology, Hunan UniversityDepartment of Cardiology, The First Hospital of Hunan University of Chinese Medicine, Branch of National Clinical Research Center for Chinese Medicine CardiologyAbstract PCSK9, which is closely related to atherosclerosis, is significantly expressed in vascular smooth muscle cells (VSMCs). Moreover, Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) mediated phenotypic transformation, abnormal proliferation, and migration of VSMCs play key roles in accelerating atherosclerosis. In this study, by utilizing the significant advantages of nano-materials, a biomimetic nanoliposome loading with Evolocumab (Evol), a PCSK9 inhibitor, was designed to alleviate atherosclerosis. In vitro results showed that (Lipo + M)@E NPs up-regulated the levels of α-SMA and Vimentin, while inhibiting the expression of OPN, which finally result in the inhibition of the phenotypic transition, excessive proliferation, and migration of VSMCs. In addition, the long circulation, excellent targeting, and accumulation performance of (Lipo + M)@E NPs significantly decreased the expression of PCSK9 in serum and VSMCs within the plaque of ApoE−/− mice.https://doi.org/10.1186/s12951-023-01904-4AtherosclerosisEvolocumabProprotein convertase subtilisin/Kexin type 9LiposomePhenotypic transition |
| spellingShingle | Zhenxian Li Haimei Zhu Hao Liu Dayue Liu Jianhe Liu Jiazheng Jiang Yi Zhang Zhang Qin Yijia Xu Yuan Peng Bin Liu Yun Long Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy Journal of Nanobiotechnology Atherosclerosis Evolocumab Proprotein convertase subtilisin/Kexin type 9 Liposome Phenotypic transition |
| title | Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy |
| title_full | Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy |
| title_fullStr | Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy |
| title_full_unstemmed | Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy |
| title_short | Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy |
| title_sort | evolocumab loaded bio liposomes for efficient atherosclerosis therapy |
| topic | Atherosclerosis Evolocumab Proprotein convertase subtilisin/Kexin type 9 Liposome Phenotypic transition |
| url | https://doi.org/10.1186/s12951-023-01904-4 |
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