Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors?

Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors?

M4 muscarinic acetylcholine receptor is a G protein-coupled receptor (GPCR) that has been associated with alcohol and cocaine abuse, Alzheimer's disease, and schizophrenia which makes it an interesting drug target. For many GPCRs, the high-affinity fluorescence ligands have expanded the options...

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Bibliographic Details
Main Authors: Maris-Johanna Tahk, Jane Torp, Mohammed A. S. Ali, Dmytro Fishman, Leopold Parts, Lukas Grätz, Christoph Müller, Max Keller, Santa Veiksina, Tõnis Laasfeld, Ago Rinken
Format: Article
Language:English
Published: The Royal Society 2022-06-01
Series:Open Biology
Subjects:
fluorescence anisotropy
microscopy
G protein-coupled receptor
muscarinic acetylcholine M4 receptor
fluorescent ligands
deep learning
Online Access:https://royalsocietypublishing.org/doi/10.1098/rsob.220019

Internet

https://royalsocietypublishing.org/doi/10.1098/rsob.220019

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