A sensitive non-radioactive <it>in situ</it> hybridization method for the detection of chicken IgG &ggr;-chain mRNA: a technique suitable for detecting of variety of mRNAs in tissue sections

<p>We established a sensitive non-radioactive <it>in situ</it> hybridization (ISH) method for the detection of chicken IgG &ggr;-chain mRNA in paraffin sections. RNA probes were transcribed <it>in vitro</it> from cloned chicken IgG CH1 nucleotide sequences with SP6/...

Full description

Bibliographic Details
Main Authors: Zheng Weiming, Izaki Junichi, Furusawa Shuichi, Yoshimura Yukinori
Format: Article
Language:English
Published: BMC 2001-01-01
Series:Biological Procedures Online
Subjects:
Online Access:http://www.biologicalprocedures.com/bpo/arts/1/18/m18.htm