A sensitive non-radioactive <it>in situ</it> hybridization method for the detection of chicken IgG &ggr;-chain mRNA: a technique suitable for detecting of variety of mRNAs in tissue sections
<p>We established a sensitive non-radioactive <it>in situ</it> hybridization (ISH) method for the detection of chicken IgG &ggr;-chain mRNA in paraffin sections. RNA probes were transcribed <it>in vitro</it> from cloned chicken IgG CH1 nucleotide sequences with SP6/...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2001-01-01
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Series: | Biological Procedures Online |
Subjects: | |
Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/18/m18.htm |