PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILS
Chicken meat represents an importance source of animal derived proteins, vitamins, and minerals. However, chicken meat might act as a potential source of human exposure to foodborne pathogens such as Staphylococcus aureus (S. aureus) and Salmonella species. The objectives of the present study were...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2021-12-01
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| Series: | Slovenian Veterinary Research |
| Subjects: | |
| Online Access: | https://www.slovetres.si/index.php/SVR/article/view/1449 |
| _version_ | 1827294460288958464 |
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| author | Amina H.A. Habashy Waiel M. Salah El-Dien Mohamed A. M. Hussein Wageh Sobhy Darwish |
| author_facet | Amina H.A. Habashy Waiel M. Salah El-Dien Mohamed A. M. Hussein Wageh Sobhy Darwish |
| author_sort | Amina H.A. Habashy |
| collection | DOAJ |
| description |
Chicken meat represents an importance source of animal derived proteins, vitamins, and minerals. However, chicken meat might act as a potential source of human exposure to foodborne pathogens such as Staphylococcus aureus (S. aureus) and Salmonella species. The objectives of the present study were first to investigate the prevalence rates of S. aureus and Salmonella species in the retailed chicken breast and thigh muscles at Zagazig city, Egypt. Second, serological identification of the isolated bacteria was followed. Third, screening of S. aureus enterotoxin coding genes (sea, seb, sec, sed, and see) and methicillin resistance (mecA) gene, as well as Salmonella virulence associated genes including Salmonella hyper-invasive locus (hilA), and Salmonella enterotoxin (stn) was done using PCR. Thereafter, the inhibitory effects of Nigella sativa, and rosemary essential oils were investigated against S. aureus, and Salmonella Typhimurium. The obtained results revealed isolation of S. aureus from the examined breast and thigh muscles at percentages of 23.3%, and 26.6%, respectively, whereas percentages of Salmonella spp. isolation from both samples were 33.3%, and 16.6%, respectively. Four Salmonella serotypes namely, S. Typhimurium, S. Enteritidis, S. Kentucky, and S. Anatum were further identified. The recovery rates of S. Typhimurium from breast and thigh muscles were 23.33% and 13.33%, respectively followed by S. Enteritidis (3.33% each). Staphylococcus enterotoxin genes (sea, and see), and mecA gene were detected in S. aureus isolates. Besides, Salmonella hilA and stn genes were also detected in Salmonella Typhimurium isolates. Nigella sativa and rosemary essential oils at 0.1%, and 0.5% could significantly reduce S. aureus, and Salmonella Typhimurium in chicken breast meat.
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| first_indexed | 2024-04-24T14:02:31Z |
| format | Article |
| id | doaj.art-b4482367f7f44e52bd9535d2d0fa807a |
| institution | Directory Open Access Journal |
| issn | 1580-4003 2385-8761 |
| language | English |
| last_indexed | 2024-04-24T14:02:31Z |
| publishDate | 2021-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Slovenian Veterinary Research |
| spelling | doaj.art-b4482367f7f44e52bd9535d2d0fa807a2024-04-03T12:38:23ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Slovenian Veterinary Research1580-40032385-87612021-12-015824-Suppl10.26873/SVR-1449-2021PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILSAmina H.A. Habashy0Waiel M. Salah El-Dien1Mohamed A. M. Hussein2Wageh Sobhy Darwish3Food Control Department, Animal Health Research Institute, Zagazig branch, Facultyculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, EgyptFood Control Department, Animal Health Research Institute, Zagazig branch, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia,44511, EgyptFood Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia, 44511, EgyptFood Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia ,44511, Egypt Chicken meat represents an importance source of animal derived proteins, vitamins, and minerals. However, chicken meat might act as a potential source of human exposure to foodborne pathogens such as Staphylococcus aureus (S. aureus) and Salmonella species. The objectives of the present study were first to investigate the prevalence rates of S. aureus and Salmonella species in the retailed chicken breast and thigh muscles at Zagazig city, Egypt. Second, serological identification of the isolated bacteria was followed. Third, screening of S. aureus enterotoxin coding genes (sea, seb, sec, sed, and see) and methicillin resistance (mecA) gene, as well as Salmonella virulence associated genes including Salmonella hyper-invasive locus (hilA), and Salmonella enterotoxin (stn) was done using PCR. Thereafter, the inhibitory effects of Nigella sativa, and rosemary essential oils were investigated against S. aureus, and Salmonella Typhimurium. The obtained results revealed isolation of S. aureus from the examined breast and thigh muscles at percentages of 23.3%, and 26.6%, respectively, whereas percentages of Salmonella spp. isolation from both samples were 33.3%, and 16.6%, respectively. Four Salmonella serotypes namely, S. Typhimurium, S. Enteritidis, S. Kentucky, and S. Anatum were further identified. The recovery rates of S. Typhimurium from breast and thigh muscles were 23.33% and 13.33%, respectively followed by S. Enteritidis (3.33% each). Staphylococcus enterotoxin genes (sea, and see), and mecA gene were detected in S. aureus isolates. Besides, Salmonella hilA and stn genes were also detected in Salmonella Typhimurium isolates. Nigella sativa and rosemary essential oils at 0.1%, and 0.5% could significantly reduce S. aureus, and Salmonella Typhimurium in chicken breast meat. https://www.slovetres.si/index.php/SVR/article/view/1449hicken musclesStaphylococcus aureusSalmonella speciesNigella sativarosemary essen-tial oils |
| spellingShingle | Amina H.A. Habashy Waiel M. Salah El-Dien Mohamed A. M. Hussein Wageh Sobhy Darwish PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILS Slovenian Veterinary Research hicken muscles Staphylococcus aureus Salmonella species Nigella sativa rosemary essen-tial oils |
| title | PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILS |
| title_full | PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILS |
| title_fullStr | PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILS |
| title_full_unstemmed | PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILS |
| title_short | PREVALENCE OF STAPHYLOCOCCUS AUREUS AND SALMONELLA SPECIES IN RETAILED CHICKEN MEAT WITH A REDUCTION TRIAL USING NIGELLA SATIVA AND ROSEMARY ESSENTIAL OILS |
| title_sort | prevalence of staphylococcus aureus and salmonella species in retailed chicken meat with a reduction trial using nigella sativa and rosemary essential oils |
| topic | hicken muscles Staphylococcus aureus Salmonella species Nigella sativa rosemary essen-tial oils |
| url | https://www.slovetres.si/index.php/SVR/article/view/1449 |
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