Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution
Oguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-020-01499-8 |
| _version_ | 1818842989046267904 |
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| author | Yusuke Oguchi Hirofumi Shintaku Sotaro Uemura |
| author_facet | Yusuke Oguchi Hirofumi Shintaku Sotaro Uemura |
| author_sort | Yusuke Oguchi |
| collection | DOAJ |
| description | Oguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method that can be applied spatial proteomics as well as spatial transcriptomics. |
| first_indexed | 2024-12-19T04:50:44Z |
| format | Article |
| id | doaj.art-b454774d269543398ffbe96da1e86973 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-19T04:50:44Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-b454774d269543398ffbe96da1e869732022-12-21T20:35:22ZengNature PortfolioCommunications Biology2399-36422020-12-013111210.1038/s42003-020-01499-8Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolutionYusuke Oguchi0Hirofumi Shintaku1Sotaro Uemura2Department of Biological Sciences, Graduate School of Science, The University of TokyoRIKEN Cluster for Pioneering ResearchDepartment of Biological Sciences, Graduate School of Science, The University of TokyoOguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method that can be applied spatial proteomics as well as spatial transcriptomics.https://doi.org/10.1038/s42003-020-01499-8 |
| spellingShingle | Yusuke Oguchi Hirofumi Shintaku Sotaro Uemura Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution Communications Biology |
| title | Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution |
| title_full | Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution |
| title_fullStr | Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution |
| title_full_unstemmed | Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution |
| title_short | Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution |
| title_sort | development of a sequencing system for spatial decoding of dna barcode molecules at single molecule resolution |
| url | https://doi.org/10.1038/s42003-020-01499-8 |
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