Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9

Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9

Cynomolgus monkey ES (Cyn ES) cells can be generated in a similar manner as human ES cells. However, Cyn ES cells are difficult to maintain in an undifferentiated state by untrained researchers. For easier culture, we generated an OCT3/4-P2A tdTomato IRES ZeocinR Cyn ES cell line using CRISPR/Cas9 g...

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Bibliographic Details
Main Authors: Kenichi Kobayashi, Tomoyuki Tsukiyama, Masataka Nakaya, Susumu Kageyama, Keiji Tomita, Ryosuke Murai, Tetsuya Yoshida, Mitsuhiro Narita, Akihiro Kawauchi, Masatsugu Ema
Format: Article
Language:English
Published: Elsevier 2019-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119300698

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http://www.sciencedirect.com/science/article/pii/S1873506119300698

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