Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16.
Genetic and biochemical analyses of human papillomavirus type 16 (HPV16) capsids have shown that certain conserved L1 cysteine residues are critical for capsid assembly, integrity, and maturation. Since previous studies utilized HPV capsids produced in monolayer culture-based protein expression syst...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3139651?pdf=render |
| _version_ | 1828412387762372608 |
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| author | Michael J Conway Linda Cruz Samina Alam Neil D Christensen Craig Meyers |
| author_facet | Michael J Conway Linda Cruz Samina Alam Neil D Christensen Craig Meyers |
| author_sort | Michael J Conway |
| collection | DOAJ |
| description | Genetic and biochemical analyses of human papillomavirus type 16 (HPV16) capsids have shown that certain conserved L1 cysteine residues are critical for capsid assembly, integrity, and maturation. Since previous studies utilized HPV capsids produced in monolayer culture-based protein expression systems, the ascribed roles for these cysteine residues were not placed in the temporal context of the natural host environment for HPV, stratifying and differentiating human tissue. Here we extend upon previous observation, that HPV16 capsids mature and become stabilized over time (10-day to 20-day) in a naturally occurring tissue-spanning redox gradient, by identifying temporal roles for individual L1 cysteine residues. Specifically, the C175S substitution severely undermined wild-type titers of the virus within both 10 and 20-day tissue, while C428S, C185S, and C175,185S substitutions severely undermined wild-type titers only within 20-day tissue. All mutations led to 20-day virions that were less stable than wild-type and failed to form L1 multimers via nonreducing SDS-PAGE. Furthermore, Optiprep-fractionated 20-day C428S, C175S, and C175,185S capsids appeared permeable to endonucleases in comparison to wild-type and C185S capsids. Exposure to an oxidizing environment failed to enhance infectious titers of any of the cysteine mutants over time as with wild-type. Introduction of these cys mutants results in failure of the virus to mature. |
| first_indexed | 2024-12-10T12:48:15Z |
| format | Article |
| id | doaj.art-b45a5cba05084fc0bd5fcb432b153712 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T12:48:15Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b45a5cba05084fc0bd5fcb432b1537122022-12-22T01:48:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2242710.1371/journal.pone.0022427Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16.Michael J ConwayLinda CruzSamina AlamNeil D ChristensenCraig MeyersGenetic and biochemical analyses of human papillomavirus type 16 (HPV16) capsids have shown that certain conserved L1 cysteine residues are critical for capsid assembly, integrity, and maturation. Since previous studies utilized HPV capsids produced in monolayer culture-based protein expression systems, the ascribed roles for these cysteine residues were not placed in the temporal context of the natural host environment for HPV, stratifying and differentiating human tissue. Here we extend upon previous observation, that HPV16 capsids mature and become stabilized over time (10-day to 20-day) in a naturally occurring tissue-spanning redox gradient, by identifying temporal roles for individual L1 cysteine residues. Specifically, the C175S substitution severely undermined wild-type titers of the virus within both 10 and 20-day tissue, while C428S, C185S, and C175,185S substitutions severely undermined wild-type titers only within 20-day tissue. All mutations led to 20-day virions that were less stable than wild-type and failed to form L1 multimers via nonreducing SDS-PAGE. Furthermore, Optiprep-fractionated 20-day C428S, C175S, and C175,185S capsids appeared permeable to endonucleases in comparison to wild-type and C185S capsids. Exposure to an oxidizing environment failed to enhance infectious titers of any of the cysteine mutants over time as with wild-type. Introduction of these cys mutants results in failure of the virus to mature.http://europepmc.org/articles/PMC3139651?pdf=render |
| spellingShingle | Michael J Conway Linda Cruz Samina Alam Neil D Christensen Craig Meyers Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16. PLoS ONE |
| title | Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16. |
| title_full | Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16. |
| title_fullStr | Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16. |
| title_full_unstemmed | Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16. |
| title_short | Differentiation-dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16. |
| title_sort | differentiation dependent interpentameric disulfide bond stabilizes native human papillomavirus type 16 |
| url | http://europepmc.org/articles/PMC3139651?pdf=render |
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