Protocol for establishing primary human lung organoid-derived air-liquid interface cultures from cryopreserved human lung tissue

Protocol for establishing primary human lung organoid-derived air-liquid interface cultures from cryopreserved human lung tissue

Summary: Primary human lung organoid-derived air-liquid interface (ALI) cultures serve as a physiologically relevant model to study human airway epithelium in vitro. Here, we present a protocol for establishing these cultures from cryopreserved human lung tissue. We describe steps for lung tissue cr...

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Bibliographic Details
Main Authors: Diana Cadena Castaneda, Sonia Jangra, Marina Yurieva, Jan Martinek, Megan Callender, Matthew Coxe, Angela Choi, Juan García-Bernalt Diego, Te-Chia Wu, Florentina Marches, Damien Chaussabel, Adolfo García-Sastre, Michael Schotsaert, Adam Williams, Karolina Palucka
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Immunology
Microscopy
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007025

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http://www.sciencedirect.com/science/article/pii/S2666166723007025

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