A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components
ABSTRACTThe simple and rapid identification of meat via DNA-based methods is still subject to the conditions of complex and time-consuming preprocessing. It usually takes 3–4 h to obtain DNA sample using conventionally available DNA extraction kits. The existing approaches are cumbersome, require la...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/09540105.2023.2248412 |
| _version_ | 1827630161797840896 |
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| author | Weilun Hu Mengxin Xu Qingya Zeng Yingying Li Yuxin Zhang Qingqing Wang Tao Ma |
| author_facet | Weilun Hu Mengxin Xu Qingya Zeng Yingying Li Yuxin Zhang Qingqing Wang Tao Ma |
| author_sort | Weilun Hu |
| collection | DOAJ |
| description | ABSTRACTThe simple and rapid identification of meat via DNA-based methods is still subject to the conditions of complex and time-consuming preprocessing. It usually takes 3–4 h to obtain DNA sample using conventionally available DNA extraction kits. The existing approaches are cumbersome, require large equipment, and are not suitable for field operation. This study developed a swelling microneedle patch, constituted by micron-sized copolymers of methyl vinyl ether and maleic acid (PMVE/MA). The microneedle patch was applied to the meat sample for 1 min before being pulled out and rinsed with Tris-EDTA (TE) buffer to obtain DNA, which could be used for polymerase chain reaction (PCR) without purification. The DNA extracted by the microneedle patch could meet the needs for amplification and identification of meat samples. The developed approach which combines the patch-based extraction method and conventional PCR amplification is quick, simple, and reliable for DNA extraction and identification of meat samples. |
| first_indexed | 2024-03-09T14:02:14Z |
| format | Article |
| id | doaj.art-b46a1d1b6c034264a3cfebd8394b13ba |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-03-09T14:02:14Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-b46a1d1b6c034264a3cfebd8394b13ba2023-11-30T06:57:34ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432023-12-0134110.1080/09540105.2023.2248412A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated componentsWeilun Hu0Mengxin Xu1Qingya Zeng2Yingying Li3Yuxin Zhang4Qingqing Wang5Tao Ma6School of Pharmacy, Bengbu Medical College, Bengbu, People’s Republic of ChinaSchool of Pharmacy, Bengbu Medical College, Bengbu, People’s Republic of ChinaSchool of Pharmacy, Bengbu Medical College, Bengbu, People’s Republic of ChinaSchool of Pharmacy, Bengbu Medical College, Bengbu, People’s Republic of ChinaSchool of Laboratory Medicine, Bengbu Medical College, Bengbu, People’s Republic of ChinaSchool of Pharmacy, Bengbu Medical College, Bengbu, People’s Republic of ChinaSchool of Pharmacy, Bengbu Medical College, Bengbu, People’s Republic of ChinaABSTRACTThe simple and rapid identification of meat via DNA-based methods is still subject to the conditions of complex and time-consuming preprocessing. It usually takes 3–4 h to obtain DNA sample using conventionally available DNA extraction kits. The existing approaches are cumbersome, require large equipment, and are not suitable for field operation. This study developed a swelling microneedle patch, constituted by micron-sized copolymers of methyl vinyl ether and maleic acid (PMVE/MA). The microneedle patch was applied to the meat sample for 1 min before being pulled out and rinsed with Tris-EDTA (TE) buffer to obtain DNA, which could be used for polymerase chain reaction (PCR) without purification. The DNA extracted by the microneedle patch could meet the needs for amplification and identification of meat samples. The developed approach which combines the patch-based extraction method and conventional PCR amplification is quick, simple, and reliable for DNA extraction and identification of meat samples.https://www.tandfonline.com/doi/10.1080/09540105.2023.2248412Microneedle patchmeatDNA extractionDNA analysisspecies identificationPCR |
| spellingShingle | Weilun Hu Mengxin Xu Qingya Zeng Yingying Li Yuxin Zhang Qingqing Wang Tao Ma A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components Food and Agricultural Immunology Microneedle patch meat DNA extraction DNA analysis species identification PCR |
| title | A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components |
| title_full | A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components |
| title_fullStr | A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components |
| title_full_unstemmed | A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components |
| title_short | A simple and rapid DNA extraction method from meat by microneedle patch for detection of adulterated components |
| title_sort | simple and rapid dna extraction method from meat by microneedle patch for detection of adulterated components |
| topic | Microneedle patch meat DNA extraction DNA analysis species identification PCR |
| url | https://www.tandfonline.com/doi/10.1080/09540105.2023.2248412 |
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