Large-Scale Preparation of Highly Stable Recombinant Human Acidic Fibroblast Growth Factor in Escherichia coli BL21(DE3) plysS Strain

Large-Scale Preparation of Highly Stable Recombinant Human Acidic Fibroblast Growth Factor in Escherichia coli BL21(DE3) plysS Strain

In this study, the optimum human aFGF gene encoding haFGF135 was cloned in pET3c and transferred to Escherichia coli BL21(DE3) plysS. To enhance the yield of fermentation and the expression level of the target protein, the fermentation parameters, including temperature, pH, dissolved oxygen, glucose...

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Bibliographic Details
Main Authors: Bingjie Yu, Wenzhe Sun, Zhen Huang, Gang Sun, Le Li, Jiawei Gu, Mengying Zheng, Xiaokun Li, ChangJu Chun, Qi Hui, Xiaojie Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
rhaFGF135
Escherichia coli BL21(DE3)plysS
fermentation
purification
wound healing
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2021.641505/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2021.641505/full

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