Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus

Generation of heterozygous (MRli003-A-5) and homozygous (MRli003-A-6) voltage-sensing knock-in human iPSC lines by CRISPR/Cas9 editing of the AAVS1 locus

Assessment of the electrophysiological properties of cardiomyocytes is necessary for phenotyping cardiac disorders and for drug screening. Optical action potential imaging using a genetically encoded voltage-sensing fluorescent protein (VSFP) allows for high-throughput functional characterization of...

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Bibliographic Details
Main Authors: Fangfang Zhang, Anna B. Meier, Daniel Sinnecker, Stefan Engelhardt, Peter Lipp, Karl-Ludwig Laugwitz, Tatjana Dorn, Alessandra Moretti
Format: Article
Language:English
Published: Elsevier 2022-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506122001349

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http://www.sciencedirect.com/science/article/pii/S1873506122001349

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