CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout

CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout

The receptor tyrosine kinase c-KIT (CD117) has a key role in hematopoiesis and is a marker for endothelial and cardiac progenitor cells. In vivo, deficiency of c-KIT is lethal and therefore using CRISPR/Cas9 editing we generated heterozygous and homozygous c-KIT knockout human embryonic stem cell (E...

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Bibliographic Details
Main Authors: Marcelo A.S. de Toledo, Xuhuang Fu, Frederick Kluge, Katrin Götz, Susanne Schmitz, Paul Wanek, Herdit M. Schüler, Kristina Pannen, Nicolas Chatain, Steffen Koschmieder, Tim H. Brümmendorf, Martin Zenke
Format: Article
Language:English
Published: Elsevier 2022-04-01
Series:Stem Cell Research
Subjects:
Embryonic stem cells
ES cells
c-KIT
CD117
Stem cell factor receptor
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506122000812

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http://www.sciencedirect.com/science/article/pii/S1873506122000812

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