Nanoparticles engineered to bind cellular motors for efficient delivery
Abstract Background Dynein is a cytoskeletal molecular motor protein that transports cellular cargoes along microtubules. Biomimetic synthetic peptides designed to bind dynein have been shown to acquire dynamic properties such as cell accumulation and active intra- and inter-cellular motion through...
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| Format: | Article |
| Language: | English |
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BMC
2018-03-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-018-0354-1 |
| _version_ | 1811298109326622720 |
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| author | Inmaculada Dalmau-Mena Pablo del Pino Beatriz Pelaz Miguel Ángel Cuesta-Geijo Inmaculada Galindo María Moros Jesús M. de la Fuente Covadonga Alonso |
| author_facet | Inmaculada Dalmau-Mena Pablo del Pino Beatriz Pelaz Miguel Ángel Cuesta-Geijo Inmaculada Galindo María Moros Jesús M. de la Fuente Covadonga Alonso |
| author_sort | Inmaculada Dalmau-Mena |
| collection | DOAJ |
| description | Abstract Background Dynein is a cytoskeletal molecular motor protein that transports cellular cargoes along microtubules. Biomimetic synthetic peptides designed to bind dynein have been shown to acquire dynamic properties such as cell accumulation and active intra- and inter-cellular motion through cell-to-cell contacts and projections to distant cells. On the basis of these properties dynein-binding peptides could be used to functionalize nanoparticles for drug delivery applications. Results Here, we show that gold nanoparticles modified with dynein-binding delivery sequences become mobile, powered by molecular motor proteins. Modified nanoparticles showed dynamic properties, such as travelling the cytosol, crossing intracellular barriers and shuttling the nuclear membrane. Furthermore, nanoparticles were transported from one cell to another through cell-to-cell contacts and quickly spread to distant cells through cell projections. Conclusions The capacity of these motor-bound nanoparticles to spread to many cells and increasing cellular retention, thus avoiding losses and allowing lower dosage, could make them candidate carriers for drug delivery. |
| first_indexed | 2024-04-13T06:14:47Z |
| format | Article |
| id | doaj.art-b4c9a787c6df4285944e2bb56d388639 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T06:14:47Z |
| publishDate | 2018-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-b4c9a787c6df4285944e2bb56d3886392022-12-22T02:58:54ZengBMCJournal of Nanobiotechnology1477-31552018-03-0116111310.1186/s12951-018-0354-1Nanoparticles engineered to bind cellular motors for efficient deliveryInmaculada Dalmau-Mena0Pablo del Pino1Beatriz Pelaz2Miguel Ángel Cuesta-Geijo3Inmaculada Galindo4María Moros5Jesús M. de la Fuente6Covadonga Alonso7Dpt. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Instituto de Nanociencia de Aragón, Universidad de ZaragozaInstituto de Nanociencia de Aragón, Universidad de ZaragozaDpt. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Dpt. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Instituto de Nanociencia de Aragón, Universidad de ZaragozaAragon Materials Science Institute (ICMA), CSIC-University of Zaragoza and CIBER-BBNDpt. Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Abstract Background Dynein is a cytoskeletal molecular motor protein that transports cellular cargoes along microtubules. Biomimetic synthetic peptides designed to bind dynein have been shown to acquire dynamic properties such as cell accumulation and active intra- and inter-cellular motion through cell-to-cell contacts and projections to distant cells. On the basis of these properties dynein-binding peptides could be used to functionalize nanoparticles for drug delivery applications. Results Here, we show that gold nanoparticles modified with dynein-binding delivery sequences become mobile, powered by molecular motor proteins. Modified nanoparticles showed dynamic properties, such as travelling the cytosol, crossing intracellular barriers and shuttling the nuclear membrane. Furthermore, nanoparticles were transported from one cell to another through cell-to-cell contacts and quickly spread to distant cells through cell projections. Conclusions The capacity of these motor-bound nanoparticles to spread to many cells and increasing cellular retention, thus avoiding losses and allowing lower dosage, could make them candidate carriers for drug delivery.http://link.springer.com/article/10.1186/s12951-018-0354-1NanoparticlesBiomimetic synthetic peptidesDrug deliveryMicrotubule motorsDynein |
| spellingShingle | Inmaculada Dalmau-Mena Pablo del Pino Beatriz Pelaz Miguel Ángel Cuesta-Geijo Inmaculada Galindo María Moros Jesús M. de la Fuente Covadonga Alonso Nanoparticles engineered to bind cellular motors for efficient delivery Journal of Nanobiotechnology Nanoparticles Biomimetic synthetic peptides Drug delivery Microtubule motors Dynein |
| title | Nanoparticles engineered to bind cellular motors for efficient delivery |
| title_full | Nanoparticles engineered to bind cellular motors for efficient delivery |
| title_fullStr | Nanoparticles engineered to bind cellular motors for efficient delivery |
| title_full_unstemmed | Nanoparticles engineered to bind cellular motors for efficient delivery |
| title_short | Nanoparticles engineered to bind cellular motors for efficient delivery |
| title_sort | nanoparticles engineered to bind cellular motors for efficient delivery |
| topic | Nanoparticles Biomimetic synthetic peptides Drug delivery Microtubule motors Dynein |
| url | http://link.springer.com/article/10.1186/s12951-018-0354-1 |
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