Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin

Abstract Two-photon microscopy enables in vivo imaging of neuronal activity in mammalian brains at high resolution. However, two-photon imaging tools for stable, long-term, and simultaneous study of multiple brain regions in same mice are lacking. Here, we propose a method to create large cranial wi...

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Bibliographic Details
Main Authors: Taiga Takahashi, Hong Zhang, Masakazu Agetsuma, Junichi Nabekura, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto
Format: Article
Language:English
Published: Nature Portfolio 2024-03-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-024-05865-8