Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin
Abstract Two-photon microscopy enables in vivo imaging of neuronal activity in mammalian brains at high resolution. However, two-photon imaging tools for stable, long-term, and simultaneous study of multiple brain regions in same mice are lacking. Here, we propose a method to create large cranial wi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-03-01
|
| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-05865-8 |
| _version_ | 1797273549510017024 |
|---|---|
| author | Taiga Takahashi Hong Zhang Masakazu Agetsuma Junichi Nabekura Kohei Otomo Yosuke Okamura Tomomi Nemoto |
| author_facet | Taiga Takahashi Hong Zhang Masakazu Agetsuma Junichi Nabekura Kohei Otomo Yosuke Okamura Tomomi Nemoto |
| author_sort | Taiga Takahashi |
| collection | DOAJ |
| description | Abstract Two-photon microscopy enables in vivo imaging of neuronal activity in mammalian brains at high resolution. However, two-photon imaging tools for stable, long-term, and simultaneous study of multiple brain regions in same mice are lacking. Here, we propose a method to create large cranial windows covering such as the whole parietal cortex and cerebellum in mice using fluoropolymer nanosheets covered with light-curable resin (termed the ‘Nanosheet Incorporated into light-curable REsin’ or NIRE method). NIRE method can produce cranial windows conforming the curved cortical and cerebellar surfaces, without motion artifacts in awake mice, and maintain transparency for >5 months. In addition, we demonstrate that NIRE method can be used for in vivo two-photon imaging of neuronal ensembles, individual neurons and subcellular structures such as dendritic spines. The NIRE method can facilitate in vivo large-scale analysis of heretofore inaccessible neural processes, such as the neuroplastic changes associated with maturation, learning and neural pathogenesis. |
| first_indexed | 2024-03-07T14:45:59Z |
| format | Article |
| id | doaj.art-b4d156fa52514f4697e3b16b9cffff18 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-07T14:45:59Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-b4d156fa52514f4697e3b16b9cffff182024-03-05T19:59:52ZengNature PortfolioCommunications Biology2399-36422024-03-017111310.1038/s42003-024-05865-8Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resinTaiga Takahashi0Hong Zhang1Masakazu Agetsuma2Junichi Nabekura3Kohei Otomo4Yosuke Okamura5Tomomi Nemoto6Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural SciencesMicro/Nano Technology Center, Tokai UniversityDivision of Homeostatic Development, National Institute for Physiological Sciences, National Institutes of Natural SciencesSchool of Life Science, The Graduate University for Advanced Studies (SOKENDAI)Division of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural SciencesMicro/Nano Technology Center, Tokai UniversityDivision of Biophotonics, National Institute for Physiological Sciences, National Institutes of Natural SciencesAbstract Two-photon microscopy enables in vivo imaging of neuronal activity in mammalian brains at high resolution. However, two-photon imaging tools for stable, long-term, and simultaneous study of multiple brain regions in same mice are lacking. Here, we propose a method to create large cranial windows covering such as the whole parietal cortex and cerebellum in mice using fluoropolymer nanosheets covered with light-curable resin (termed the ‘Nanosheet Incorporated into light-curable REsin’ or NIRE method). NIRE method can produce cranial windows conforming the curved cortical and cerebellar surfaces, without motion artifacts in awake mice, and maintain transparency for >5 months. In addition, we demonstrate that NIRE method can be used for in vivo two-photon imaging of neuronal ensembles, individual neurons and subcellular structures such as dendritic spines. The NIRE method can facilitate in vivo large-scale analysis of heretofore inaccessible neural processes, such as the neuroplastic changes associated with maturation, learning and neural pathogenesis.https://doi.org/10.1038/s42003-024-05865-8 |
| spellingShingle | Taiga Takahashi Hong Zhang Masakazu Agetsuma Junichi Nabekura Kohei Otomo Yosuke Okamura Tomomi Nemoto Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin Communications Biology |
| title | Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin |
| title_full | Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin |
| title_fullStr | Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin |
| title_full_unstemmed | Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin |
| title_short | Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin |
| title_sort | large scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light curable resin |
| url | https://doi.org/10.1038/s42003-024-05865-8 |
| work_keys_str_mv | AT taigatakahashi largescalecranialwindowforinvivomousebrainimagingutilizingfluoropolymernanosheetandlightcurableresin AT hongzhang largescalecranialwindowforinvivomousebrainimagingutilizingfluoropolymernanosheetandlightcurableresin AT masakazuagetsuma largescalecranialwindowforinvivomousebrainimagingutilizingfluoropolymernanosheetandlightcurableresin AT junichinabekura largescalecranialwindowforinvivomousebrainimagingutilizingfluoropolymernanosheetandlightcurableresin AT koheiotomo largescalecranialwindowforinvivomousebrainimagingutilizingfluoropolymernanosheetandlightcurableresin AT yosukeokamura largescalecranialwindowforinvivomousebrainimagingutilizingfluoropolymernanosheetandlightcurableresin AT tomominemoto largescalecranialwindowforinvivomousebrainimagingutilizingfluoropolymernanosheetandlightcurableresin |