Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin
Abstract Two-photon microscopy enables in vivo imaging of neuronal activity in mammalian brains at high resolution. However, two-photon imaging tools for stable, long-term, and simultaneous study of multiple brain regions in same mice are lacking. Here, we propose a method to create large cranial wi...
Main Authors: | Taiga Takahashi, Hong Zhang, Masakazu Agetsuma, Junichi Nabekura, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2024-03-01
|
Series: | Communications Biology |
Online Access: | https://doi.org/10.1038/s42003-024-05865-8 |
Similar Items
-
PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain
by: Taiga Takahashi, et al.
Published: (2020-10-01) -
Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain
by: Taiga Takahashi, et al.
Published: (2021-06-01) -
Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution.
by: Hong Zhang, et al.
Published: (2020-01-01) -
Real-Time Polarization-Resolved Imaging of Living Tissues Based on Two-Photon Excitation Spinning-Disk Confocal Microscopy
by: Ai Goto, et al.
Published: (2019-04-01) -
Programmable UV-Curable Resin by Dielectric Force
by: Yi-Wei Lin, et al.
Published: (2023-02-01)