Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies
Introduction: Skeletal muscle is a major contributor to whole-body energy homeostasis and the utilization of fatty acids and glucose. At present, 2D cell models have been the most used cellular models to study skeletal muscle energy metabolism. However, the transferability of the results to in vivo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1130693/full |
| _version_ | 1797862058173136896 |
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| author | Andrea Dalmao-Fernandez Aleksandra Aizenshtadt Hege G. Bakke Stefan Krauss Arild C. Rustan G. Hege Thoresen G. Hege Thoresen Eili Tranheim Kase |
| author_facet | Andrea Dalmao-Fernandez Aleksandra Aizenshtadt Hege G. Bakke Stefan Krauss Arild C. Rustan G. Hege Thoresen G. Hege Thoresen Eili Tranheim Kase |
| author_sort | Andrea Dalmao-Fernandez |
| collection | DOAJ |
| description | Introduction: Skeletal muscle is a major contributor to whole-body energy homeostasis and the utilization of fatty acids and glucose. At present, 2D cell models have been the most used cellular models to study skeletal muscle energy metabolism. However, the transferability of the results to in vivo might be limited. This project aimed to develop and characterize a skeletal muscle 3D cell model (myospheres) as an easy and low-cost tool to study molecular mechanisms of energy metabolism.Methods and results: We demonstrated that human primary myoblasts form myospheres without external matrix support and carry structural and molecular characteristics of mature skeletal muscle after 10 days of differentiation. We found significant metabolic differences between the 2D myotubes model and myospheres. In particular, myospheres showed increased lipid oxidative metabolism than the 2D myotubes model, which oxidized relatively more glucose and accumulated more oleic acid.Discussion and conclusion: These analyses demonstrate model differences that can have an impact and should be taken into consideration for studying energy metabolism and metabolic disorders in skeletal muscle. |
| first_indexed | 2024-04-09T22:14:21Z |
| format | Article |
| id | doaj.art-b4dd0506ab1d4dd487b3cdad5fa28ab5 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-09T22:14:21Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b4dd0506ab1d4dd487b3cdad5fa28ab52023-03-23T06:02:33ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-03-011110.3389/fbioe.2023.11306931130693Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studiesAndrea Dalmao-Fernandez0Aleksandra Aizenshtadt1Hege G. Bakke2Stefan Krauss3Arild C. Rustan4G. Hege Thoresen5G. Hege Thoresen6Eili Tranheim Kase7Section for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Oslo, NorwayHybrid Technology Hub Centre of Excellence, Faculty of Medicine, University of Oslo, Oslo, NorwaySection for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Oslo, NorwayHybrid Technology Hub Centre of Excellence, Faculty of Medicine, University of Oslo, Oslo, NorwaySection for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Oslo, NorwaySection for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Oslo, NorwayDepartment of Pharmacology, Institute of Clinical Medicine, University of Oslo, Oslo, NorwaySection for Pharmacology and Pharmaceutical Biosciences, Department of Pharmacy, University of Oslo, Oslo, NorwayIntroduction: Skeletal muscle is a major contributor to whole-body energy homeostasis and the utilization of fatty acids and glucose. At present, 2D cell models have been the most used cellular models to study skeletal muscle energy metabolism. However, the transferability of the results to in vivo might be limited. This project aimed to develop and characterize a skeletal muscle 3D cell model (myospheres) as an easy and low-cost tool to study molecular mechanisms of energy metabolism.Methods and results: We demonstrated that human primary myoblasts form myospheres without external matrix support and carry structural and molecular characteristics of mature skeletal muscle after 10 days of differentiation. We found significant metabolic differences between the 2D myotubes model and myospheres. In particular, myospheres showed increased lipid oxidative metabolism than the 2D myotubes model, which oxidized relatively more glucose and accumulated more oleic acid.Discussion and conclusion: These analyses demonstrate model differences that can have an impact and should be taken into consideration for studying energy metabolism and metabolic disorders in skeletal muscle.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1130693/fullskeletal musclemyosphereenergy metabolismmetabolic disorders3D cell modelmuscle spheroid |
| spellingShingle | Andrea Dalmao-Fernandez Aleksandra Aizenshtadt Hege G. Bakke Stefan Krauss Arild C. Rustan G. Hege Thoresen G. Hege Thoresen Eili Tranheim Kase Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies Frontiers in Bioengineering and Biotechnology skeletal muscle myosphere energy metabolism metabolic disorders 3D cell model muscle spheroid |
| title | Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies |
| title_full | Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies |
| title_fullStr | Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies |
| title_full_unstemmed | Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies |
| title_short | Development of three-dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies |
| title_sort | development of three dimensional primary human myospheres as culture model of skeletal muscle cells for metabolic studies |
| topic | skeletal muscle myosphere energy metabolism metabolic disorders 3D cell model muscle spheroid |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1130693/full |
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