Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gondii</named-content>

Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gondii</named-content>

ABSTRACT CRISPR-Cas9 technologies have enabled genome engineering in an unprecedented array of species, accelerating biological studies in both model and nonmodel systems. However, Cas9 can be inherently toxic, which has limited its use in some organisms. We previously described the serendipitous di...

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Bibliographic Details
Main Authors: Benedikt M. Markus, George W. Bell, Hernan A. Lorenzi, Sebastian Lourido
Format: Article
Language:English
Published: American Society for Microbiology 2019-06-01
Series:mSphere
Subjects:
CRISPR
Cas9
Toxoplasma gondii
genome editing
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00386-19

Internet

https://journals.asm.org/doi/10.1128/mSphere.00386-19

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