Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu tea
This study aimed to accurately identify the “golden flower” fungus, and characterize its effects on the chemical composition of Fu tea. A “golden flower” fungus strain was isolated from Hunan Fu tea and named as ACF-1. The ACF-1 was identified as Aspergillus cristatus using culture characteristics,...
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| Format: | Article |
| Language: | zho |
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Science Press
2021-10-01
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| Series: | Fushe yanjiu yu fushe gongyi xuebao |
| Subjects: | |
| Online Access: | http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2021.rrj.39.050701&lang=zh |
| _version_ | 1797900509413113856 |
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| author | RAN Lisha LIU Baogui CHEN Chongjun TANG Qian ZHANG Zhang ZHU Mingzhi FU Donghe WANG Kunbo |
| author_facet | RAN Lisha LIU Baogui CHEN Chongjun TANG Qian ZHANG Zhang ZHU Mingzhi FU Donghe WANG Kunbo |
| author_sort | RAN Lisha |
| collection | DOAJ |
| description | This study aimed to accurately identify the “golden flower” fungus, and characterize its effects on the chemical composition of Fu tea. A “golden flower” fungus strain was isolated from Hunan Fu tea and named as ACF-1. The ACF-1 was identified as Aspergillus cristatus using culture characteristics, optical microscopic structure, electron microscopic structure, ITS region sequence alignment analysis, and multigene phylogeny analysis based on β-tubulin, calmodulin, and RNA polymerase Ⅱ gene sequences. ACF-1 fungus was inoculated into sterilized primary dark tea for a loose tea “flowering” experiment, to analyze changes in the main chemical components. Polyphenol, free amino acid, soluble sugar, and flavonoid contents changed substantially in the “flowering” samples compared to non-“flowering”, decreasing by 11.3%, 17.3%, 11.9%, and 33.8%, respectively. The variation of chemical components made a foundation for the quality improvement during the process. These findings provide a more comprehensive understanding of the formation of the sensory characteristics in Fu tea and provide a guidance for the development and utilization of “golden flower” fungus. |
| first_indexed | 2024-04-10T08:47:13Z |
| format | Article |
| id | doaj.art-b5158d14e1dd4bf4b8a1492a1040da83 |
| institution | Directory Open Access Journal |
| issn | 1000-3436 |
| language | zho |
| last_indexed | 2024-04-10T08:47:13Z |
| publishDate | 2021-10-01 |
| publisher | Science Press |
| record_format | Article |
| series | Fushe yanjiu yu fushe gongyi xuebao |
| spelling | doaj.art-b5158d14e1dd4bf4b8a1492a1040da832023-02-22T07:22:42ZzhoScience PressFushe yanjiu yu fushe gongyi xuebao1000-34362021-10-01395929910.11889/j.1000-3436.2021.rrj.39.0507011000-3436（2021）05-0092-08Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu teaRAN Lisha0LIU Baogui1CHEN Chongjun2TANG Qian3ZHANG Zhang4ZHU Mingzhi5FU Donghe6WANG Kunbo7Key Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, ChinaChina Tea (Hunan) Anhua 1st Factory Co., Ltd., Anhua 413500, ChinaKey Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Ministry of Education for Tea Science, Hunan Agricultural University, Changsha 410128, ChinaThis study aimed to accurately identify the “golden flower” fungus, and characterize its effects on the chemical composition of Fu tea. A “golden flower” fungus strain was isolated from Hunan Fu tea and named as ACF-1. The ACF-1 was identified as Aspergillus cristatus using culture characteristics, optical microscopic structure, electron microscopic structure, ITS region sequence alignment analysis, and multigene phylogeny analysis based on β-tubulin, calmodulin, and RNA polymerase Ⅱ gene sequences. ACF-1 fungus was inoculated into sterilized primary dark tea for a loose tea “flowering” experiment, to analyze changes in the main chemical components. Polyphenol, free amino acid, soluble sugar, and flavonoid contents changed substantially in the “flowering” samples compared to non-“flowering”, decreasing by 11.3%, 17.3%, 11.9%, and 33.8%, respectively. The variation of chemical components made a foundation for the quality improvement during the process. These findings provide a more comprehensive understanding of the formation of the sensory characteristics in Fu tea and provide a guidance for the development and utilization of “golden flower” fungus.http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2021.rrj.39.050701&lang=zhaspergillus cristatusidentificationpolygenic alignment modifiedartificial inoculationchemical composition |
| spellingShingle | RAN Lisha LIU Baogui CHEN Chongjun TANG Qian ZHANG Zhang ZHU Mingzhi FU Donghe WANG Kunbo Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu tea Fushe yanjiu yu fushe gongyi xuebao aspergillus cristatus identification polygenic alignment modified artificial inoculation chemical composition |
| title | Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu tea |
| title_full | Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu tea |
| title_fullStr | Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu tea |
| title_full_unstemmed | Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu tea |
| title_short | Isolation and identification of “golden flower” fungus and determination of its effects on chemical the composition of Fu tea |
| title_sort | isolation and identification of golden flower fungus and determination of its effects on chemical the composition of fu tea |
| topic | aspergillus cristatus identification polygenic alignment modified artificial inoculation chemical composition |
| url | http://www.fs.sinap.ac.cn/thesisDetails#10.11889/j.1000-3436.2021.rrj.39.050701&lang=zh |
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