Characterization of Protocatechuate 4,5-Dioxygenase from <i>Pseudarthrobacter phenanthrenivorans</i> Sphe3 and In Situ Reaction Monitoring in the NMR Tube

Characterization of Protocatechuate 4,5-Dioxygenase from <i>Pseudarthrobacter phenanthrenivorans</i> Sphe3 and In Situ Reaction Monitoring in the NMR Tube

The current study aims at the functional and kinetic characterization of protocatechuate (PCA) 4,5-dioxygenase (PcaA) from <i>Pseudarthrobacter phenanthrenivorans</i> Sphe3. This is the first single subunit Type II dioxygenase characterized in Actinobacteria. RT-PCR analysis demonstrated...

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Bibliographic Details
Main Authors: Epameinondas Tsagogiannis, Elpiniki Vandera, Alexandra Primikyri, Stamatia Asimakoula, Andreas G. Tzakos, Ioannis P. Gerothanassis, Anna-Irini Koukkou
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Pseudarthrobacter phenanthrenivorans</i> Sphe3
protocatechuate (PCA) 4,5-dioxygenase
in situ biotransformation monitoring
NMR bioreactor
extradiol dioxygenases
Actinobacteria
Online Access:https://www.mdpi.com/1422-0067/22/17/9647

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https://www.mdpi.com/1422-0067/22/17/9647

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