Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mannooligosaccharides production from galactomannans

Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mannooligosaccharides production from galactomannans

In this study, an acidophilic GH5 β-mannanase (TaMan5) from Trichoderma asperellum ND-1 was efficiently expressed in Pichia pastoris (a 2.0-fold increase, 67.5 ± 1.95 U/mL). TaMan5 displayed the highest specificity toward locust bean gum (Km = 1.34 mg/mL, Vmax = 749.14 μmol/min/mg) at pH 4.0 and 65°...

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Bibliographic Details
Main Authors: Fengzhen Zheng, Abdul Basit, Jiaqiang Wang, Huan Zhuang, Jun Chen, Jianfen Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Microbiology
Subjects:
Trichoderma asperellum
β-mannanase
enzyme characteristics
action model
synergism
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1191553/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2023.1191553/full

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