Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology

Optimization of a recombinant BlaR-CTD protein formulation using the response surface methodology

Abstract The sequence of a carboxy-terminal of the β-lactam sensor-transducer protein (BlaR-CTD) from Bacillus licheniformis ATCC14580 was extracted from US7745193B2 patent and expressed in E. coli using pColdI vector as a soluble His-tag recombinant protein. In this study, several excipients were u...

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Bibliographic Details
Main Authors: Mohadeseh Haji Abdolvahab, Mojdeh Safari, Farkhonde Hasannejad, Nika Asefi, Alireza Salimi, Mahboobeh Nazari
Format: Article
Language:English
Published: BMC 2024-01-01
Series:Journal of Biological Engineering
Subjects:
β-Lactam antibiotics
BlaR-CTD
Stability
RSM
CCD
Design of Experiments
Online Access:https://doi.org/10.1186/s13036-023-00399-9

Internet

https://doi.org/10.1186/s13036-023-00399-9

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