Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system
ABSTRACT Background: The venom of Phoneutria nigriventer spider is a source of numerous bioactive substances, including some toxins active in insects. An example is PnTx4(5-5) that shows a high insecticidal activity and no apparent toxicity to mice, although it inhibited NMDA-evoked currents in ra...
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| Format: | Article |
| Language: | English |
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100316&lng=en&tlng=en |
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| author | Camila Franco Batista Oliveira Daniela Pereira Alves Bruna Luiza Emerich Suely Gomes de Figueiredo Marta do Nascimento Cordeiro Márcia Helena Borges Michael Richardson Adriano Monteiro de Castro Pimenta Igor Dimitri Gama Duarte Maria Elena de Lima |
| author_facet | Camila Franco Batista Oliveira Daniela Pereira Alves Bruna Luiza Emerich Suely Gomes de Figueiredo Marta do Nascimento Cordeiro Márcia Helena Borges Michael Richardson Adriano Monteiro de Castro Pimenta Igor Dimitri Gama Duarte Maria Elena de Lima |
| author_sort | Camila Franco Batista Oliveira |
| collection | DOAJ |
| description | ABSTRACT Background: The venom of Phoneutria nigriventer spider is a source of numerous bioactive substances, including some toxins active in insects. An example is PnTx4(5-5) that shows a high insecticidal activity and no apparent toxicity to mice, although it inhibited NMDA-evoked currents in rat hippocampal neurons. In this work the analgesic activity of PnTx4(5-5) (renamed Γ-ctenitoxin-Pn1a) was investigated. Methods: The antinociceptive activity was evaluated using the paw pressure test in rats, after hyperalgesia induction with intraplantar injection of carrageenan or prostaglandin E2 (PGE2). Results: PnTx4(5-5), subcutaneously injected, was able to reduce the hyperalgesia induced by PGE2 in rat paw, demonstrating a systemic effect. PnTx4(5-5) administered in the plantar surface of the paw caused a peripheral and dose-dependent antinociceptive effect on hyperalgesia induced by carrageenan or PGE2. The hyperalgesic effect observed in these two pain models was completely reversed with 5 µg of PnTx4(5-5). Intraplantar administration of L-glutamate induced hyperalgesic effect that was significantly reverted by 5 μg of PnTx4(5-5) injection in rat paw. Conclusion: The antinociceptive effect for PnTx4(5-5) was demonstrated against different rat pain models, i.e. induced by PGE2, carrageenan or glutamate. We suggest that the antinociceptive effect of PnTx4(5-5) may be related to an inhibitory activity on the glutamatergic system. |
| first_indexed | 2024-12-12T22:33:15Z |
| format | Article |
| id | doaj.art-b5934e9134ef402f9f4f875bd0a62013 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-12T22:33:15Z |
| publisher | SciELO |
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| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-b5934e9134ef402f9f4f875bd0a620132022-12-22T00:09:32ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992510.1590/1678-9199-jvatitd-2019-0022S1678-91992019000100316Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic systemCamila Franco Batista OliveiraDaniela Pereira AlvesBruna Luiza EmerichSuely Gomes de FigueiredoMarta do Nascimento CordeiroMárcia Helena BorgesMichael RichardsonAdriano Monteiro de Castro PimentaIgor Dimitri Gama DuarteMaria Elena de LimaABSTRACT Background: The venom of Phoneutria nigriventer spider is a source of numerous bioactive substances, including some toxins active in insects. An example is PnTx4(5-5) that shows a high insecticidal activity and no apparent toxicity to mice, although it inhibited NMDA-evoked currents in rat hippocampal neurons. In this work the analgesic activity of PnTx4(5-5) (renamed Γ-ctenitoxin-Pn1a) was investigated. Methods: The antinociceptive activity was evaluated using the paw pressure test in rats, after hyperalgesia induction with intraplantar injection of carrageenan or prostaglandin E2 (PGE2). Results: PnTx4(5-5), subcutaneously injected, was able to reduce the hyperalgesia induced by PGE2 in rat paw, demonstrating a systemic effect. PnTx4(5-5) administered in the plantar surface of the paw caused a peripheral and dose-dependent antinociceptive effect on hyperalgesia induced by carrageenan or PGE2. The hyperalgesic effect observed in these two pain models was completely reversed with 5 µg of PnTx4(5-5). Intraplantar administration of L-glutamate induced hyperalgesic effect that was significantly reverted by 5 μg of PnTx4(5-5) injection in rat paw. Conclusion: The antinociceptive effect for PnTx4(5-5) was demonstrated against different rat pain models, i.e. induced by PGE2, carrageenan or glutamate. We suggest that the antinociceptive effect of PnTx4(5-5) may be related to an inhibitory activity on the glutamatergic system.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100316&lng=en&tlng=enspider toxinΓ-ctenitoxin-Pn1aPnTx4(5-5)Phoneutria nigriventerAntinociceptionglutamate |
| spellingShingle | Camila Franco Batista Oliveira Daniela Pereira Alves Bruna Luiza Emerich Suely Gomes de Figueiredo Marta do Nascimento Cordeiro Márcia Helena Borges Michael Richardson Adriano Monteiro de Castro Pimenta Igor Dimitri Gama Duarte Maria Elena de Lima Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system Journal of Venomous Animals and Toxins including Tropical Diseases spider toxin Γ-ctenitoxin-Pn1a PnTx4(5-5) Phoneutria nigriventer Antinociception glutamate |
| title | Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system |
| title_full | Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system |
| title_fullStr | Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system |
| title_full_unstemmed | Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system |
| title_short | Antinociceptive effect of PnTx4(5-5), a peptide from Phoneutria nigriventer spider venom, in rat models and the involvement of glutamatergic system |
| title_sort | antinociceptive effect of pntx4 5 5 a peptide from phoneutria nigriventer spider venom in rat models and the involvement of glutamatergic system |
| topic | spider toxin Γ-ctenitoxin-Pn1a PnTx4(5-5) Phoneutria nigriventer Antinociception glutamate |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100316&lng=en&tlng=en |
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