Dynamics of ribosomes and release factors during translation termination in E. coli

Dynamics of ribosomes and release factors during translation termination in E. coli

Release factors RF1 and RF2 promote hydrolysis of peptidyl-tRNA during translation termination. The GTPase RF3 promotes recycling of RF1 and RF2. Using single molecule FRET and biochemical assays, we show that ribosome termination complexes that carry two factors, RF1–RF3 or RF2–RF3, are dynamic and...

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Bibliographic Details
Main Authors: Sarah Adio, Heena Sharma, Tamara Senyushkina, Prajwal Karki, Cristina Maracci, Ingo Wohlgemuth, Wolf Holtkamp, Frank Peske, Marina V Rodnina
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-06-01
Series:eLife
Subjects:
ribosome
smFRET
conformational dynamics
GTP hydrolysis
protein synthesis
translation termination
Online Access:https://elifesciences.org/articles/34252

Internet

https://elifesciences.org/articles/34252

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