A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9

A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9

Until recently, the favored method for making directed modifications to the budding yeast genome involved the introduction of a DNA template carrying the desired genetic changes along with a selectable marker, flanked by homology arms. This approach both limited the ability to make changes within ge...

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Bibliographic Details
Main Authors: Rhiannon R. Aguilar, Zih-Jie Shen, Jessica K. Tyler
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Methods and Protocols
Subjects:
CRISPR-Cas9
budding yeast
homologous recombination
point mutation
genome editing
genetic engineering
Online Access:https://www.mdpi.com/2409-9279/5/5/79

Internet

https://www.mdpi.com/2409-9279/5/5/79

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