Site-Specific Protein Modifications by an Engineered Asparaginyl Endopeptidase from Viola canadensis

Site-Specific Protein Modifications by an Engineered Asparaginyl Endopeptidase from Viola canadensis

Asparaginyl endopeptidases (AEPs) or legumains are Asn/Asp (Asx)-specific proteases that break peptide bonds, but also function as peptide asparaginyl ligases (PALs) that make peptide bonds. This ligase activity can be used for site-specific protein modifications in biochemical and biotechnological...

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Bibliographic Details
Main Authors: Yu Chen, Dingpeng Zhang, Xiaohong Zhang, Zhen Wang, Chuan-Fa Liu, James P. Tam
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-10-01
Series:Frontiers in Chemistry
Subjects:
asparaginyl endopeptidases
peptide asparaginyl ligases
ligase-activity determinant
Viola canadensis
cell surface labeling
protein engineering
Online Access:https://www.frontiersin.org/articles/10.3389/fchem.2021.768854/full

Internet

https://www.frontiersin.org/articles/10.3389/fchem.2021.768854/full

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