Engineering of <i>Escherichia coli</i> Glyceraldehyde-3-Phosphate Dehydrogenase with Dual NAD<sup>+</sup>/NADP<sup>+</sup> Cofactor Specificity for Improving Amino Acid Production
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the central metabolism of microbial cells. GAPDHs differ in cofactor specificity and use NAD<sup>+</sup>, NADP<sup>+</sup>, or both cofactors, reducing them to NADH and NADPH, respectively. Sufficient NADPH s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/5/976 |