Effect of the Kozak sequence on transient expression of human coagulation factor IX (FIX) minigene in mammalian cells

Effect of the Kozak sequence on transient expression of human coagulation factor IX (FIX) minigene in mammalian cells

Background: The positive effect of Kozak sequence on the translational efficiency of the eukaryotic genes has been noted and can be used in order to increase recombinant protein expression. Methods: A CMV-regulated human factor IX (hFIX) –expressing plasmid containing factor IX minigene– equipped w...

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Bibliographic Details
Main Authors: Aliakbar Hadad Mashhadrizeh, Mohamad reza Saam, Sedigheh Safary, Alireza Zomorodi pour, Seyed Javad Hosseini, farzaneh Sabooni
Format: Article
Language:English
Published: Bushehr University of Medical Sciences 2009-02-01
Series:Iranian South Medical Journal
Subjects:
Kozak sequence
eukaryotic genes expression
recombinant protein
factor IX
Online Access:http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-136&slc_lang=en&sid=1

Internet

http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-136&slc_lang=en&sid=1

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