A nonspecific primer anchored PCR technique for chromosome walking
A chromosome walking method was improved in this work. The new method was named as nonspecific primer anchored PCR (NPA-PCR). Nested gene specific primers were designed based on the known region and long random primer using degeneracy oligonucleotides for nonspecific anchoring. Annealing temperature...
| Príomhchruthaitheoirí: | , , , , |
|---|---|
| Formáid: | Alt |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
Instituto de Tecnologia do Paraná (Tecpar)
2011-02-01
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| Sraith: | Brazilian Archives of Biology and Technology |
| Ábhair: | |
| Rochtain ar líne: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100013 |