Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS
The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to d4 are allergens present in a variety of different isoforms in apples. In this study...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Food Chemistry: Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566222000399 |
| _version_ | 1818211406341734400 |
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| author | Soraya Chebib Chen Meng Christina Ludwig Karl-Christian Bergmann Sylvia Becker Werner Dierend Wilfried Schwab |
| author_facet | Soraya Chebib Chen Meng Christina Ludwig Karl-Christian Bergmann Sylvia Becker Werner Dierend Wilfried Schwab |
| author_sort | Soraya Chebib |
| collection | DOAJ |
| description | The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to d4 are allergens present in a variety of different isoforms in apples. In this study, we used proteomics to quantify all four Mal d proteins in 52 apple genotypes with varying allergenic potentials. A total of 195, 17, 14, and 18 peptides were found to be related to Mal d1, d2, d3, and d4 proteins, respectively of which 25 different Mal d proteins could be unambiguously identified. The allergenic potential of the Mal d isoforms was characterized by comparing the isoform abundance with the allergenic score of genotypes from oral challenge tests. The detected Mal d peptides presumably have different IgE binding properties and could be used as potential molecular markers to discriminate between hypoallergenic and hyperallergenic cultivars. |
| first_indexed | 2024-12-12T05:32:00Z |
| format | Article |
| id | doaj.art-b647cfdaf1b6435fb055c3b15d1c0c99 |
| institution | Directory Open Access Journal |
| issn | 2666-5662 |
| language | English |
| last_indexed | 2024-12-12T05:32:00Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj.art-b647cfdaf1b6435fb055c3b15d1c0c992022-12-22T00:36:17ZengElsevierFood Chemistry: Molecular Sciences2666-56622022-07-014100111Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MSSoraya Chebib0Chen Meng1Christina Ludwig2Karl-Christian Bergmann3Sylvia Becker4Werner Dierend5Wilfried Schwab6Biotechnology of Natural Products, Technical University Munich, Liesel-Beckmann-Str. 1, 85354 Freising, GermanyBavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University Munich, Gregor-Mendel-Straße 4, 85354 Freising, GermanyBavarian Center for Biomolecular Mass Spectrometry (BayBioMS), Technical University Munich, Gregor-Mendel-Straße 4, 85354 Freising, GermanyAllergy-Centre-Charité, Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, GermanyEuropean Centre Allergy Research Foundation, Robert-Koch-Platz 7, 10115 Berlin, GermanyFaculty of Agricultural Science and Landscape Architecture, Fruit Science, University of Applied Sciences Osnabrück, Oldenburger Landstr. 24, 49090 Osnabrück, GermanyBiotechnology of Natural Products, Technical University Munich, Liesel-Beckmann-Str. 1, 85354 Freising, Germany; Corresponding author.The apple fruit (Malus domestica L. Borkh) is one of the most popular fruits worldwide. Beyond their beneficial properties, apples contain proteins that trigger allergic reactions in susceptible consumers. Mal d1 to d4 are allergens present in a variety of different isoforms in apples. In this study, we used proteomics to quantify all four Mal d proteins in 52 apple genotypes with varying allergenic potentials. A total of 195, 17, 14, and 18 peptides were found to be related to Mal d1, d2, d3, and d4 proteins, respectively of which 25 different Mal d proteins could be unambiguously identified. The allergenic potential of the Mal d isoforms was characterized by comparing the isoform abundance with the allergenic score of genotypes from oral challenge tests. The detected Mal d peptides presumably have different IgE binding properties and could be used as potential molecular markers to discriminate between hypoallergenic and hyperallergenic cultivars.http://www.sciencedirect.com/science/article/pii/S2666566222000399Malus domesticaMal d proteinsProteomicsLC-MS/MSIsoformsApple breeding |
| spellingShingle | Soraya Chebib Chen Meng Christina Ludwig Karl-Christian Bergmann Sylvia Becker Werner Dierend Wilfried Schwab Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS Food Chemistry: Molecular Sciences Malus domestica Mal d proteins Proteomics LC-MS/MS Isoforms Apple breeding |
| title | Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS |
| title_full | Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS |
| title_fullStr | Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS |
| title_full_unstemmed | Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS |
| title_short | Identification of allergenomic signatures in allergic and well-tolerated apple genotypes using LC-MS/MS |
| title_sort | identification of allergenomic signatures in allergic and well tolerated apple genotypes using lc ms ms |
| topic | Malus domestica Mal d proteins Proteomics LC-MS/MS Isoforms Apple breeding |
| url | http://www.sciencedirect.com/science/article/pii/S2666566222000399 |
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